Enzymes

Inorganic pyrophosphate (PPi) is generated as a reaction byproduct in many biosynthetic reactions which utilize ATP, including in vitro transcription and DNA polymerization. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate (P₂O₇^-4 + H₂O + PPase 2HPO₄^-2). PPase requires divalent metal cation (Mg²⁺) for the enzymatic activity.

RNase III is ribonuclease III (RNase III) recombinantly expressed by E. coli. This specific exonuclease cleaves double-stranded RNA (dsRNA) and generates 12-35bp dsRNA fragments with 5'-PO4 and 3'-OH, 3' overhangs.

RNase III is ribonuclease III (RNase III) recombinantly expressed by E. coli. This specific exonuclease cleaves double-stranded RNA (dsRNA) and generates 12-35bp dsRNA fragments with 5'-PO4 and 3'-OH, 3' overhangs.

T7 RNA Polymerase is a DNA-dependent RNA polymerase from T7 phage, that possesses a strong and specific 5' to 3' RNA polymerase activity. T7 RNA Polymerase has high specificity for T7 promoter sequences and will synthesize large quantities of RNA from a DNA fragment inserted downstream from a promoter.

T7 RNA Polymerase is a DNA-dependent RNA polymerase from T7 phage, that possesses a strong and specific 5' to 3' RNA polymerase activity. T7 RNA Polymerase has high specificity for T7 promoter sequences and will synthesize large quantities of RNA from a DNA fragment inserted downstream from a promoter.

T7 RNA Polymerase is a DNA-dependent RNA polymerase from T7 phage, that possesses a strong and specific 5' to 3' RNA polymerase activity. T7 RNA Polymerase has high specificity for T7 promoter sequences and will synthesize large quantities of RNA from a DNA fragment inserted downstream from a promoter.

Inorganic Pyrophosphatase (Yeast) is derived from a recombinant <i>E.Coli</i> strain carrying <i>Saccharomyces cerevisiae</i> ppa gene. The enzyme catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate. P₂O₇^-4 + H₂O + PPase 2HPO₄^-2 Inorganic Pyrophosphatase (yeast) could hydrolyze the inorganic pyrophosphate generated by nucleic acid amplification, in vitro transcription and other reactions, removing the inhibition of the inorganic pyrophosphate generated on the reaction system. The removal of pyrophosphate can shift the reaction equilibrium to the end of product formation, which is beneficial to increase the yield of synthetic product. RNA and DNA synthesis are examples of reactions that can be pulled far in the synthesis direction by the action of inorganic pyrophosphatase.

Inorganic Pyrophosphatase (Yeast) is derived from a recombinant <i>E.Coli</i> strain carrying <i>Saccharomyces cerevisiae</i> ppa gene. The enzyme catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate. P₂O₇^-4 + H₂O + PPase 2HPO₄^-2 Inorganic Pyrophosphatase (yeast) could hydrolyze the inorganic pyrophosphate generated by nucleic acid amplification, in vitro transcription and other reactions, removing the inhibition of the inorganic pyrophosphate generated on the reaction system. The removal of pyrophosphate can shift the reaction equilibrium to the end of product formation, which is beneficial to increase the yield of synthetic product. RNA and DNA synthesis are examples of reactions that can be pulled far in the synthesis direction by the action of inorganic pyrophosphatase.

Inorganic Pyrophosphatase (Yeast) is derived from a recombinant <i>E.Coli</i> strain carrying <i>Saccharomyces cerevisiae</i> ppa gene. The enzyme catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate. P₂O₇^-4 + H₂O + PPase 2HPO₄^-2 Inorganic Pyrophosphatase (yeast) could hydrolyze the inorganic pyrophosphate generated by nucleic acid amplification, in vitro transcription and other reactions, removing the inhibition of the inorganic pyrophosphate generated on the reaction system. The removal of pyrophosphate can shift the reaction equilibrium to the end of product formation, which is beneficial to increase the yield of synthetic product. RNA and DNA synthesis are examples of reactions that can be pulled far in the synthesis direction by the action of inorganic pyrophosphatase.