Enzymes

Poly(A) Polymerase is a polymerase expressed by recombinant <i>E.coli</i>. The enzyme does not depend on the template and can catalyze the sequential incorporation of ATP into the 3' end of RNA in the form of AMP, that is, adding a polyadenosine tail to the 3' end of RNA. Poly(A) structure can improve the stability of RNA and improve the translation efficiency of mRNA in eukaryotic cells. Poly(A) polymerase has high tailing efficiency and can add 20-200 A bases to the 3' end of RNA.

T4 RNA Ligase is an ATP-dependent T4 RNA ligase (T4 RNA Ligase I) recombinantly expressed by <i>E.coli</i>. This enzyme catalyzes the formation of phosphodiester bonds between oligonucleotides, single-stranded RNA and DNA intermolecular/intra-molecular 5'-PO4 and 3'-OH.

T4 RNA Ligase is an ATP-dependent T4 RNA ligase (T4 RNA Ligase I) recombinantly expressed by <i>E.coli</i>. This enzyme catalyzes the formation of phosphodiester bonds between oligonucleotides, single-stranded RNA and DNA intermolecular/intra-molecular 5'-PO4 and 3'-OH.

Alkaline Phosphatase is derived from a recombinant <i>E.coli</i> strain that carries the TAB5 gene. The enzyme catalyzes the dephosphorylation of 5' and 3' ends of DNA and RNA phosphomonoesters. Also, it hydrolyses ribose, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). TAB5 Alkaline Phosphatase acts on 5´ protruding, 5' recessed and blunt ends. The Phosphatase can be used in many molecular biology applications, such as cloning or probe end labeling to remove the phosphorylated ends of DNA or RNA. In cloning experiments, dephosphorylation prevents the linearized plasmid DNA from self-ligation. It can also degrade unincorporated dNTPs in PCR reactions to prepare a template for DNA sequencing. The enzyme is completely and irreversibly inactivated by heating at 70&deg;C for 5 minutes, thereby making removal of the phosphatase prior to ligation or end labeling unnecessary.

Alkaline Phosphatase is derived from a recombinant <i>E.coli</i> strain that carries the TAB5 gene. The enzyme catalyzes the dephosphorylation of 5' and 3' ends of DNA and RNA phosphomonoesters. Also, it hydrolyses ribose, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). TAB5 Alkaline Phosphatase acts on 5´ protruding, 5' recessed and blunt ends. The Phosphatase can be used in many molecular biology applications, such as cloning or probe end labeling to remove the phosphorylated ends of DNA or RNA. In cloning experiments, dephosphorylation prevents the linearized plasmid DNA from self-ligation. It can also degrade unincorporated dNTPs in PCR reactions to prepare a template for DNA sequencing. The enzyme is completely and irreversibly inactivated by heating at 70&deg;C for 5 minutes, thereby making removal of the phosphatase prior to ligation or end labeling unnecessary.

Alkaline Phosphatase is derived from a recombinant <i>E.coli</i> strain that carries the TAB5 gene. The enzyme catalyzes the dephosphorylation of 5' and 3' ends of DNA and RNA phosphomonoesters. Also, it hydrolyses ribose, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). TAB5 Alkaline Phosphatase acts on 5´ protruding, 5' recessed and blunt ends. The Phosphatase can be used in many molecular biology applications, such as cloning or probe end labeling to remove the phosphorylated ends of DNA or RNA. In cloning experiments, dephosphorylation prevents the linearized plasmid DNA from self-ligation. It can also degrade unincorporated dNTPs in PCR reactions to prepare a template for DNA sequencing. The enzyme is completely and irreversibly inactivated by heating at 70&deg;C for 5 minutes, thereby making removal of the phosphatase prior to ligation or end labeling unnecessary.

The Bsa I restriction endonuclease is a restriction endonuclease BsaI that can recognize specific sites expressed by Escherichia coli recombinantly. Restriction endonucleases are widely used in various fields such as gene positioning and cloning. This enzyme cleaves DNA rapidly for efficient gene linearization.

The Bsa I restriction endonuclease is a restriction endonuclease BsaI that can recognize specific sites expressed by Escherichia coli recombinantly. Restriction endonucleases are widely used in various fields such as gene positioning and cloning. This enzyme cleaves DNA rapidly for efficient gene linearization.

The restriction endonuclease is a restriction endonuclease BspQ I that can recognize specific sites and is produced under GMP standards and expressed recombinantly in Escherichia coli. Restriction endonucleases are widely used in various fields such as gene positioning and cloning. This enzyme cleaves DNA rapidly for efficient gene linearization.