Enzymes

Recombinant full length human USP21 (Ubiquitin Specific Protease 21), amino acids 2-566(end). This construct contains an N-terminal His-tag (6xHis) and a C-terminal FLAG-tag. This protein was affinity purified.

Recombinant human VEGFR2 (vascular endothelial growth factor receptor 2), also known as kinase insert domain receptor (KDR), encompassing amino acids 20-764 corresponding to the extracellular domain of the receptor. This construct contains a C-terminal Avi-Tag™ followed by an His-tag (6xHis). This protein was affinity purified. 

Recombinant human VEGFR2 (vascular endothelial growth factor receptor 2), also known as kinase insert domain receptor (KDR), encompassing amino acids 20-764 corresponding to the extracellular domain of the receptor. This construct contains a C-terminal Avi-Tag™ followed by an His-tag (6xHis). This protein was affinity purified. 

Recombinant human JAK3 (Janus Kinase 3), encompassing amino acids 511-790 corresponding to the pseudokinase domain (JH2 domain) which lacks enzymatic activity. This construct contains a C-terminal Avi-Tag™ followed by a His-tag (6xHis). This protein was affinity purified.

Recombinant human JAK3 (Janus Kinase 3), encompassing amino acids 511-790 corresponding to the pseudokinase domain (JH2 domain) which lacks enzymatic activity. This construct contains a C-terminal Avi-Tag™ followed by a His-tag (6xHis). This protein was affinity purified.

Lyophilized. Dialyzed.

RNase R is an E. coli exoribonuclease which exhibits 3' to 5' exonuclease activity, efficiently digesting nearly all linear RNA species. This enzyme does not digest circular, lariat, or double stranded RNA with short 3’ overhangs (less than seven nucleotides).

Among the most important techniques in proteome analyses is the in-gel digestion of protein spots/bands that have been resolved by electrophoresis using digestive enzymes, such as trypsin and lysyl endopoptidase. Proteins can be identified by mass spectrometry analysis of the peptides produced by in-gel digestion, and further information regarding post-translational modifications can be obtained.Lysyl Endopeptidase, Mass Spectrometry Grade is a freeze dried product that retained sufficient activity for in-gel digestion and packed in very small quantities for convenience purposes.

Among the most important techniques in proteome analyses is the in-gel digestion of protein spots/bands that have been resolved by electrophoresis using digestive enzymes, such as trypsin and lysyl endopoptidase. Proteins can be identified by mass spectrometry analysis of the peptides produced by in-gel digestion, and further information regarding post-translational modific ations can be obtained.Lysyl Endopeptidase, Mass Spectrometry Grade is a freeze dried product that retained sufficient activity for in-gel digestion and packed in very small quantities for convenience purposes.