Enzymes

GenCRISPR™ LbuCas13a Nuclease is an RNA-guided RNA endonuclease from Leptotrichia buccalis. Cas13a (previously known as C2c2) belongs to the Class 2 Type VI CRISPR-Cas system and contains a single protein effector with two HEPN domains. Cas13a exhibits two distinct ribonuclease activities. Cas13a possesses sequence-specific RNA cleavage activity in vitro and in vivo. And after specific cleavage, Cas13a activates collateral cleavage activity towards adjacent non-specific ssRNA sequences. The two distinct ribonuclease activities of Cas13a enable guide-RNA processing and RNA detection. The RNA detection empowered with Cas13a-crRNA complex and quenched fluorescent ssRNA as reporter has been reported to be versatile, rapid and high-specific.

Human CD52 full-length ORF (AAH00644.1, 1 a.a. - 61 a.a.) recombinant protein with GST tag at N-terminal.

Human COX6A2 full-length ORF (NP_005196.1, 1 a.a. - 97 a.a.) recombinant protein with GST tag at N-terminal.

Human GNA12 full-length ORF (AAH09457, 1 a.a. - 98 a.a.) recombinant protein with GST tag at N-terminal.

Human GUCA2B full-length ORF (NP_009033.1, 1 a.a. - 112 a.a.) recombinant protein with GST tag at N-terminal.

Human HSPA1A full-length ORF (AAH02453, 1 a.a. - 641 a.a.) recombinant protein with GST tag at N-terminal.

Human KIFC3 full-length ORF (AAH34234.1, 1 a.a. - 687 a.a.) recombinant protein with GST tag at N-terminal.

Human MTHFR full-length ORF (AAH18766, 1 a.a. - 73 a.a.) recombinant protein with GST tag at N-terminal.

Human PER1 full-length ORF (NP_002607.1, 1 a.a. - 1290 a.a.) recombinant protein with GST tag at N-terminal.