Enzymes

Recombinant human JAK1 (janus kinase 1), encompassing amino acids 561-852 that correspond to the JH2 pseudokinase domain). This construct contains an N-terminal His-tag (6xHis). This protein was affinity purified.

Recombinant human JAK1 (janus kinase 1), encompassing amino acids 561-852 that correspond to the JH2 pseudokinase domain). This construct contains an N-terminal His-tag (6xHis). This protein was affinity purified.

Recombinant human EGFR (epidermal growth factor receptor) encompassing tyrosine kinase domain amino acids 668-1210(end) with a deletion of amino acids 746 to 750 and mutations T790M and C797S. This construct contains an N-terminal GST-tag and a C-terminal His-tag (6xHis). This protein was affinity purified.

Recombinant human KEAP1 (kelch like ECH associated protein 1), full length, encompassing amino acids 2-624. This construct contains an N-terminal His-tag (6xHis). This recombinant protein was affinity purified. 

Human Akt1 (GenBank Accession No. NM_001014432), full length (a.a. 1-480) with N-terminal His-tag, MW=60.2 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human Akt1 (GenBank Accession No. NM_001014432), full length (a.a. 1-480) with N-terminal His-tag, MW=60.2 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Recombinant human EIF4EBP1 (eukaryotic translation initiation factor 4E binding protein 1), full length, amino acids 1-118 (end). This construct contains a N-terminal His-tag (6xHis). The recombinant protein was affinity purified.

Recombinant human EIF4EBP1 (eukaryotic translation initiation factor 4E binding protein 1), full length, amino acids 1-118 (end). This construct contains a N-terminal His-tag (6xHis). The recombinant protein was affinity purified.

Human hexosaminidase B (beta polypeptide), also known as HEXB, (GenBank Accession No. NM_000521), full length (a.a. 1-556(end)) with C-terminal FLAG-His-tags, MW = 64.9 kDa, expressed in a baculovirus infected Sf9 cell expression system.