Enzymes

The kit is used for detection and quantitative determination of Ultra Nuclease in the samples.

The kit is used for detection and quantitative determination of Ultra Nuclease in the samples.

This kit is used to detect RNase contamination in sample.

This kit is used to detect RNase contamination in sample.

mRNA Cap 2'-O-methyltransferase was derived from a recombinant <i>E.coli</i> strain that carries the gene for the vaccinia mRNA Cap 2'-O-Methyltransferase. This enzyme adds a methyl group at the 2'-O position of the first nucleotide adjacent to the cap structure at the 5' end of the RNA. The enzyme utilizes S-adenosylmethionine (SAM) as a methyl donor to methylate capped RNA (cap-0) resulting in a cap-1 structure. The Cap 1 structure can increase the translation efficiency, improving the expression of mRNA in transfection and microinjection experiments. This enzyme specifically requires RNA with an m7GpppN cap as substrate. It cannot utilize RNA with pN, ppN, pppN or GpppN at the 5'end. Capped RNA may be prepared via in vitro transcription using cap analog or through enzymatic capping using the Vaccinia Capping Enzyme.

Poly(A) Polymerase is a polymerase expressed by recombinant <i>E.coli</i>. The enzyme does not depend on the template and can catalyze the sequential incorporation of ATP into the 3' end of RNA in the form of AMP, that is, adding a polyadenosine tail to the 3' end of RNA. Poly(A) structure can improve the stability of RNA and improve the translation efficiency of mRNA in eukaryotic cells. Poly(A) polymerase has high tailing efficiency and can add 20-200 A bases to the 3' end of RNA.

Alkaline Phosphatase is derived from a recombinant <i>E.coli</i> strain that carries the TAB5 gene. The enzyme catalyzes the dephosphorylation of 5' and 3' ends of DNA and RNA phosphomonoesters. Also, it hydrolyses ribose, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). TAB5 Alkaline Phosphatase acts on 5´ protruding, 5' recessed and blunt ends. The Phosphatase can be used in many molecular biology applications, such as cloning or probe end labeling to remove the phosphorylated ends of DNA or RNA. In cloning experiments, dephosphorylation prevents the linearized plasmid DNA from self-ligation. It can also degrade unincorporated dNTPs in PCR reactions to prepare a template for DNA sequencing. The enzyme is completely and irreversibly inactivated by heating at 70&deg;C for 5 minutes, thereby making removal of the phosphatase prior to ligation or end labeling unnecessary.

The restriction endonuclease is a restriction endonuclease BspQ I that can recognize specific sites and is produced under GMP standards and expressed recombinantly in Escherichia coli. Restriction endonucleases are widely used in various fields such as gene positioning and cloning. This enzyme cleaves DNA rapidly for efficient gene linearization.

Murine RNase inhibitor binds to RNase A, B or C in a 1:1 ratio through non-covalent bonding, thereby inhibiting the activity of the enzymes and protecting RNA from degradation.