Enzymes

E. coli inorganic pyrophosphatase (iPPase) catalyzes the hydrolysis of inorganic pyrophosphate (PPi) to form orthophosphate. Using iPPase in an in vitro transcription reaction drives RNA synthesis forward by removing pyrophosphate which forms as a byproduct and precipitates with key transcription buffer cations. The provided pack sizes are configured to run 25 rxns or 250 rxns of 100-μL IVT, following our recommended co-transcriptional protocol to produce Cap-1 mRNAs. Please see the details in the product insert. The 30 µg and 310 µg pack sizes contain approximately 6 units and 62 units of enzymes, respectively. Unit definition: One unit of enzyme produces 1 µmol of phosphate from inorganic pyrophosphate in 1 min at 25°C.

E. coli inorganic pyrophosphatase (iPPase) catalyzes the hydrolysis of inorganic pyrophosphate (PPi) to form orthophosphate. Using iPPase in an in vitro transcription reaction drives RNA synthesis forward by removing pyrophosphate which forms as a byproduct and precipitates with key transcription buffer cations. The provided pack sizes are configured to run 25 rxns or 250 rxns of 100-μL IVT, following our recommended co-transcriptional protocol to produce Cap-1 mRNAs. Please see the details in the product insert. The 30 µg and 310 µg pack sizes contain approximately 6 units and 62 units of enzymes, respectively. Unit definition: One unit of enzyme produces 1 µmol of phosphate from inorganic pyrophosphate in 1 min at 25°C.

Wild-type bacteriophage T7 RNA polymerase is a highly processive, 100 kDa, DNA-dependent RNA polymerase that catalyzes the in vitro transcription (IVT) of a recombinant gene regulated by the T7 promoter. It is a key enzyme used to synthesize mRNA (messenger RNA) from a DNA template. It is highly efficient and specific, capable of transcribing large quantities of RNA in a relatively short time frame. Its high robustness and reproducibility makes it the standard industry choice for mRNA synthesis as well as other applications such as saRNA synthesis, radiolabeled RNA probe preparation, and RNA construct development for additional studies. The provided pack sizes are configured to run 25 rxns or 250 rxns of 100-μL IVT, following our recommended co-transcriptional protocol to produce Cap-1 mRNAs. Please see the details in the product insert.The 100 µg and 840 µg pack sizes contain approximately 50,000 units and 420,000 units of enzymes, respectively.  Unit definition: One unit of enzy

Wild-type bacteriophage T7 RNA polymerase is a highly processive, 100 kDa, DNA-dependent RNA polymerase that catalyzes the in vitro transcription (IVT) of a recombinant gene regulated by the T7 promoter. It is a key enzyme used to synthesize mRNA (messenger RNA) from a DNA template. It is highly efficient and specific, capable of transcribing large quantities of RNA in a relatively short time frame. Its high robustness and reproducibility makes it the standard industry choice for mRNA synthesis as well as other applications such as saRNA synthesis, radiolabeled RNA probe preparation, and RNA construct development for additional studies. The provided pack sizes are configured to run 25 rxns or 250 rxns of 100-μL IVT, following our recommended co-transcriptional protocol to produce Cap-1 mRNAs. Please see the details in the product insert.The 100 µg and 840 µg pack sizes contain approximately 50,000 units and 420,000 units of enzymes, respectively.  Unit definition: One unit of enzy

Engineered RNase Inhibitor binds to ribonucleases A, B, and C in a 1:1 stoichiometry with femtomolar affinity and renders them unable to bind or degrade RNA. Engineered RNase Inhibitor has significantly improved resistance to oxidation compared to the human/porcine RNase inhibitors and is stable at low DTT concentrations. The provided pack sizes are configured to run 25 rxns or 250 rxns of 100-μL IVT, following our recommended co-transcriptional protocol to produce Cap-1 mRNAs. Please see the details in the product insert. The 60 µg and 530 µg pack sizes contain approximately 3,000 units and 26,500 units of enzymes, respectively. Unit definition: One unit of the enzyme inhibits the activity of 5 ng of RNase A by 50%.

Engineered RNase Inhibitor binds to ribonucleases A, B, and C in a 1:1 stoichiometry with femtomolar affinity and renders them unable to bind or degrade RNA. Engineered RNase Inhibitor has significantly improved resistance to oxidation compared to the human/porcine RNase inhibitors and is stable at low DTT concentrations. The provided pack sizes are configured to run 25 rxns or 250 rxns of 100-μL IVT, following our recommended co-transcriptional protocol to produce Cap-1 mRNAs. Please see the details in the product insert. The 60 µg and 530 µg pack sizes contain approximately 3,000 units and 26,500 units of enzymes, respectively. Unit definition: One unit of the enzyme inhibits the activity of 5 ng of RNase A by 50%.

TriLink’s CleanScribe™ RNA Polymerase is a novel DNA-dependent RNA polymerase that catalyzes the in vitro transcription (IVT) of a recombinant gene regulated by the T7 promoter. This enzyme reduces double-strand RNA (dsRNA) levels by up to 85% during IVT. dsRNA is a byproduct of IVT reactions and can trigger undesirable inflammatory responses in host cells.

TriLink’s CleanScribe™ RNA Polymerase is a novel DNA-dependent RNA polymerase that catalyzes the in vitro transcription (IVT) of a recombinant gene regulated by the T7 promoter. This enzyme reduces double-strand RNA (dsRNA) levels by up to 85% during IVT. dsRNA is a byproduct of IVT reactions and can trigger undesirable inflammatory responses in host cells. This novel RNA polymerase can directly substitute a wild-type T7 RNA polymerase in an IVT protocol to synthesize messenger RNAs (mRNAs) from a DNA template. It is highly efficient and specific, capable of transcribing large quantities of RNA in a relatively short time frame. Its robustness, reproducibility, and dsRNA reduction make it ideal for mRNA synthesis as well as other applications such as saRNA synthesis, radiolabeled RNA probe preparation, and RNA construct development for additional studies.

Recombinant Aeromonas Aminopeptidase (Legacy Tebubio ref. 167100-10). Proteases (also called Proteolytic Enzymes, Peptidases, or Proteinases) are enzymes that hydrolyze the amide bonds within proteins or peptides. Most proteases act in a specific manner, hydrolyzing bonds at, or adjacent to specific residues, or a specific sequence of residues contained within the substrate protein or peptide. Proteases play an important role in most diseases and biological processes, including prenatal and postnatal development, reproduction, signal transduction, the immune response, various autoimmune and degenerative diseases, and cancer. They are also an important research tool, frequently used in the analysis and production of proteins. Recombinant Aeromonas Aminopeptidase is a 31.4 kDa protein containing 291 amino acid residues.