Enzymes

Human hematopoietic progenitor kinase 1 (HPK1) also known as mitogen activated protein kinase kinase kinase kinase 1 (MAP4K1), GenBank Accession No. NM_007181, a.a. 1-346, with N-terminal GST tag, MW = 65 kDa. HPK1 is expressed in Sf9 insect cells via a baculovirus expression system.

Human Ack1 (GenBank Accession No. NM_005781), a.a. 110-476(end) with N-terminal GST-tag, MW=68 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

Recombinant full-length human BLK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST-tag. The gene accession number is BC007371.

Recombinant full-length human cytoplasmic tyrosine-protein kinase BMX (BMX), also known as bone marrow tyrosine kinase gene in chromosome X protein, epithelial and endothelial tyrosine kinase, ETK, and NTK38 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag, a.a. 1-675 (end). The gene accession number is NM_001721 (variant 2).

Human BRK, also known as PTK6 (GenBank Accession No. NM_005975), 2-451 (end), with N-terminal GST-tag, MW=80 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

Human BTK (GenBank Accession No. NM_000061), full length with N-terminal GST-tag, MW= 106 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human CSK (GenBank Accession No. NM_004383), full length with N-terminal GST-tag, MW= 80 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human Abelson murine leukemia viral oncogene homolog 1 (Abl-1) (GenBank Accession No. NM_007313), a.a. 248-531, with N-terminal His-tag, MW = 34.8 kDa, expressed in a Baculovirus-infected Sf9 cell expression system

Human Wee1 protein, also known as Wee1A Kinase, GenBank Accession No. NM_003390, amino acids 215-646 (end), with an N-terminal GST-Thrombin tag, expressed in a Baculovirus infected Sf9 cell expression system. MW = 75 kDa.