Enzymes

Human MEK1, or mitogen-activated protein kinase kinase 1 (MAP2K1) (GenBank Accession No. NM_002755), full length a.a. 2 - 393 (end), with N-terminal GST-tag, MW = 69 kDa, expressed in baculovirus-infected Sf9 cell expression system.

Human v-raf murine sarcoma viral oncogene homolog B1 (BRAF), GenBank Accession No. NM_004333, a.a. 417-end with Val-to-Glu mutation on a.a. 600* and N-terminal GST-tag and His-tag, MW=69 kDa, expressed in Sf9 insect cells via a baculovirus expression system.*This mutation is often cited as V600E.

Human myelin basic protein (MBP), GenBank Accession No. NM_001025101, a.a. 2-304(end), with N-terminal His-tag, MW=34 kDa, expressed in an E. coli cell expression system.

PI/PS (4:1, w/w) unilamellar vesicle, prepared with the sodium salts of L-α-phosphatidylinositol (Bovine liver) and L-α-phosphatidylserine (Porcine brain). Lipid monolayer was formed by sonication. MW PI= 909.12, MW PS= 812.05.

Human recombinant RB protein (773-end) with N-terminal GST-tag (GenBank Accession No. NM_000321), MW=43.5 kDa, expressed in an E. coli expression system.

Human Sphingosine kinase 1 or SphK1 (GenBank Accession No. AF200328), full length with N-terminal His-tag, MW=46.9 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human Sphingosine kinase 2 (GenBank Accession No. AF245447), full length with N-terminal His-tag, MW=69.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human Sphingosine kinase 2 (GenBank Accession No.NM_020126), full length with N-terminal His-tag, MW=74 kDa, expressed in a Baculovirus infected Sf9 cell expression system

Mouse Sphingosine kinase 1 (GenBank Accession No. AF415213), full length with N-terminal His-tag, MW=47.1 kDa, expressed in a Baculovirus infected Sf9 cell expression system.