Enzymes

Mouse Sphingosine kinase 2 (GenBank Accession No. NM_203280), full length with N-terminal His-tag, MW=68 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human ceramide kinase (CERK), GenBank Accession No. NM_022766, a.a. 1-537(end) with C-terminal FLAG-tag, MW= 60 kDa, expressed in a baculovirus expression system.

Substrate for ceramide kinase (CERK), optimized for use with β-Octylglucoside mixed micellar assay.

Substrate for ceramide kinase (CERK), optimized for use with β-Octylglucoside mixed micellar assay.

Complex of N-terminal FLAG-tagged recombinant full-length human p110α (GenBank Accession No. NM_006218), and recombinant full length, human p85α (no tag) (GenBank Accession No. NM_181523). Coexpressed in a Baculovirus-infected Sf9 cell expression system. p110α MW=125 kDa, p85α MW=84 kDa.

Complex of N-terminal GST-tagged recombinant full-length human p110α (GenBank Accession No. NM_006218), and recombinant full length, human p85α (no tag) (GenBank Accession No. NM_181523). Coexpressed in a Baculovirus infected Sf9 cell expression system. p110α MW=151 kDa, p85α MW=84 kDa.

Complex of N-terminal FLAG-tagged recombinant full-length human p110β (GenBank Accession No. NM_006219), and untagged, recombinant full length, human p85α. (GenBank Accession No. NM_181523). Coexpressed in a Baculovirus infected Sf9 cell expression system. p110β MW=124 kDa, p85α MW=84 kDa.

Human p120γ (gamma) (GenBank Accession No. NM_002649), full length with N-terminal His-tag, MW=130 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Complex of N-terminal His-tag recombinant human p110γ (PIK3CG), (GenBank Accession No. NM_002649) a.a. 2-1102 (end), and N-terminal GST-tag recombinant human PIK3R5 (GenBank Accession No. NM_001142633) a.a. 2 -880 (end). Co-expressed in a Baculovirus infected Sf9 cell expression system. p110 MW=127 kDa, PIK3R5 MW=124 kDa.