Enzymes

Complex of N-terminal GST-tagged recombinant full-length human p110δ (GenBank Accession No. NM_005026.2), and recombinant full length, human p85α, GenBank Accession No. NM_181523). Coexpressed in a Baculovirus infected Sf9 cell expression system. p110δ MW=146 kDa, p85α MW=84 kDa.

Human p85α (GenBank Accession No. NM_181523), full length with N-terminal His-tag, MW=84 kDa, expressed in an Baculovirus infected Sf9 cell expression system.

Complex of N-terminal FLAG-tagged recombinant full-length human p110α (GenBank Accession No. NM_006218), with K111N mutation, and recombinant full length, human p85α (no tag, GenBank Accession No. NM_181523). Co-expressed in Sf9 cells in a Baculovirus expression system. p110α(K111N) MW=124 kDa, p85α MW=84 kDa.

Complex of N-terminal FLAG-tagged recombinant full-length human p110α (GenBank Accession No. NM_006218), with E545K mutation, and recombinant full length, human p85α (no tag, GenBank Accession No. NM_181523). Co-expressed in Sf9 cells in a Baculovirus expression system. p110α(E545K) MW=125 kDa, p85α MW=84 kDa.

Complex of N-terminal FLAG-tagged recombinant full-length human p110α (GenBank Accession No. NM_006218), with H1047R mutation, and recombinant full length, human p85α (no tag, GenBank Accession No. NM_181523). Co-expressed in Sf9 cells in a Baculovirus expression system. p110α(H1047R) MW=125 kDa, p85α MW=84 kDa.

Human inositol hexakisphosphate kinase 2 (IP6K2), also known as IHPK2, InsP6 kinase 2, and PIUS, (GenBank Accession No. NM_016291), a.a. 1-426 (end), with C-terminal FLAG and His-tags, MW=51 kDa, expressed in baculovirus infected Sf9 cells.

Human inositol hexakisphosphate kinase 2 (IP6K2), also known as IHPK2, InsP6 kinase 2, and PIUS, (GenBank Accession No. NM_016291), a.a. 1-426 (end), with C-terminal His-tag and Avi-tag™, MW=52 kDa, expressed in an E. coli cell expression system and enzymatically biotin-labeled using Avi-tag™ technology.

Complex of recombinant full-length human p110α (GenBank Accession No. NM_006218) with E545K mutation and N-terminal His-Tag, and recombinant full length, human p85α (GenBank Accession No. NM_181523), no tag. Co-expressed in Sf9 cells in a Baculovirus expression system. p110α (E545K) MW=111 kDa, p85α MW=86 kDa.

Complex of recombinant full-length human p110α (GenBank Accession No. NM_006218), with N-terminal His-Tag and recombinant full length, human p55γ (GenBank Accession No. NM_003629.2), no tag. Co-expressed in Sf9 cells in a Baculovirus expression system. p110α MW=111 kDa, p85α MW=61 kDa.