Enzymes

Human LRRK2 protein with a Y1699G mutation, also known as PARK8, RIPK7, and ROCO2, GenBank Accession No. NM_198578, amino acids 968-end, with an N-terminal GST-tag, expressed in an SF9 cell expression system. MW = 210 kDa.

Human LRRK2, also known as PARK8, RIPK7, and ROCO2, GenBank Accession No. NM_198578, amino acids 968-end, with an N-terminal GST-tag, expressed in an SF9 cell expression system. MW = 210 kDa.

Human JNK1-β1(K55M), also known as MAPK8 (K55M) (GenBank Accession No. NM_139046), full length with mutation of lysine55 to methionine, and N-terminal GST-tag, MW=~70 kDa, expressed in E. coli. The K55M mutation prevents ATP binding, making the kinase inactive.

Mouse Cot kinase (MAP3K8), GenBank Accession No. NM_007746, a.a. 30-397, with N-terminal His-GST-tag, expressed in a Baculovirus infected Sf9 cell expression system. Theoretical MW is 70 kDa.

Complex of recombinant full-length human CDK2 (GenBank accession No. NM_001798), a.a. 1-298 (end), MW = 34 kDa with no tag, and CyclinA2 (GenBank accession No. NM_001237), a.a. 174-432 (end) with N-terminal His-GST-tags, MW = 57 kDa. Both were co-expressed in a baculovirus Sf9 insect cell expression system.

Human MLK1, also known as Mixed Lineage Kinase 1, Mitogen-activated protein kinase kinase kinase 9, MAP3K9, MEKK9, and PRKE1, GenBank Accession No. NM_033141, a.a. 1-433, with N-terminal GST-tag, MW = 77 kDa, expressed in Sf9 cells via a baculovirus expression system.

Human MLK2, also known as Mixed Lineage Kinase 2, Mitogen-activated protein kinase kinase kinase 10, MAP3K10, and MST, GenBank Accession No. NM_002446, a.a. 1-446, with N-terminal GST-tag, MW = 76 kDa, expressed in Sf9 cells via a baculovirus expression system.

Human MLK3, also known as Mixed Lineage Kinase 3, Mitogen-activated protein kinase kinase kinase 11, MAP3K11, PTK1, SPRK, and MGC17114, GenBank Accession No. NM_002419, a.a. 1-488, with N-terminal GST-tag, MW = 83 kDa, expressed in Sf9 cells via a baculovirus expression system.

Human MLK4, also known as Mixed Lineage Kinase 4, and KIAA1804, GenBank Accession No. NM_032435, a.a. 1-557, with N-terminal GST-tag, MW = 61 kDa, expressed in Sf9 cells via a baculovirus expression system.