Enzymes

Recombinant human serine/threonine-protein kinase PIM1 (PIM1), GenBank Accession No. NM_002648, full-length with an N-terminal GST tag and expressed in a baculovirus infected Sf9 insect cell expression system. MW = 62 kDa.

Recombinant human serine/threonine-protein kinase PIM3 (PIM3), GenBank Accession No. NM_145478, full-length with an N-terminal GST tag and expressed in a baculovirusinfected Sf9 insect cell expression system. MW = 63 kDa.

Recombinant human calcium/calmodulin-dependent protein kinase 2 delta (CAMK2?), also known as CAMKII?, CAMK2d and CAMKIId, GenBank Accession No. NM_172115, fulllength with an N-terminal GST tag and expressed in a baculovirus-infected Sf9 insect cell expression system. MW = 77 kDa.

Human BRAF, also known as B-RAF1 and RAFB1, with 18 mutations to improve expression; S446A, S447A, I543A, I544S, I551K, Q562R, L588N, K630S, F667E, Y672S, A688R, L706S, Q709R, S713E, L716E, S720E, P722S, K723G. GenBank Accession #NM_004333, a.a. 444-723 with N-terminal His-tag, expressed in E. coli cells. MW = 33 kDa.

Human HDAC2 , GenBank Accession No. NM_001527, full length with C-terminal His-tag, MW= 56 kDa, expressed in baculovirus expression system.

Complex of human HDAC3 , GenBank Accession No. NM_003883, full length with C-terminal His-tag, MW= 49.7 kDa, and human NCOR2 , a.a. 395- 489, GenBank Accession No. NM_006312, N-terminal GST-tag, MW= 37.6 kDa, co-expressed in baculovirus expression system.

Human HDAC4 (GenBank Accession No. NM_006037), a.a. 627-1084 (end) with N-terminal GST-tag and C-terminal His-tag, MW= 76 kDa, expressed in baculovirus expression system.

Human HDAC5 catalytic domain , GenBank Accession No. NM_005474, a.a. 656-1122 (end) with C-terminal His-tag, MW= 51 kDa, expressed in baculovirus expression system.

Human HDAC8 , GenBank Accession No. NM_018486, full length with C-terminal His-tag, MW= 46.4 kDa, expressed in a baculovirus expression system.