Enzymes

Human HDAC6 with H611A mutation (GenBank Accession No. NM_006044), full length with N-terminal GST-tag, MW= 161 kDa, expressed in a baculovirus expression system.

Human E1A binding protein p300, also known as EP300, KAT3B, or RSTS2, GenBank Accession No. NM_001429, a.a. 965-1810, with N-terminal His-GST-tag, MW=125 kDa, expressed in Sf9 cells via a baculovirus expression system.

Human alpha tubulin acetyltransferase 1, or ATAT1, GenBank Accession No. NM_001031722 (var1), a.a. 2 - 224, with N-terminal GST-tag, MW=52 kDa, expressed in an E. coli expression system.

Human GCN5, also known as KAT2A, GCNL2, or PCAF-β, GenBank Accession No. NM_021078, a.a. 362-837 (end), with C-terminal FLAG-tag, MW=55 kDa, expressed in a baculovirus infected Sf9 cell expression system.

Variant 2 Human LSD1 [also known as KDM1A (lysine (K)-specific demethylase 1A) or AOF2, amine oxidase (flavin containing) domain 2)] GenBank Accession No. NM_015013, a.a. 158-end, MW=77.9 kDa, expressed in an E. coli expression system with N-terminal GST-tag and PreScission Protease site. GST-tag was cleaved after affinity purification.

Human LSD1 [also known as KDM1A (lysine (K)-specific demethylase 1A) or AOF2, amine oxidase (flavin containing) domain 2)] GenBank Accession No. NM_015013, a.a. 158-end with N-terminal GST-tag, MW=103 kDa, expressed in an E. coli expression system.

Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No. NM_015015) (a.a. 1-500) with C-terminal FLAG-tag, MW=57 kDa, expressed in Sf9 cells using a Baculovirus expression system.

Human JMJD2C (GenBank Accession No. BC143571) (a.a. 2-372) with N-terminal GST-tag, MW=69 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human JARID1A, also known as KDM5A and RBBP2, GenBank Accession #NM_001042603), a.a. 1-1090 with C-terminal FLAG-tag, MW= 125 kDa, expressed in Sf9 cells via a baculovirus expression system.