Enzymes

Human ACC1 (acetyl-Coenzyme A carboxylase alpha transcript variant 1) (GenBank Accession No. NM_198834), a.a. 1-2383(end) with C-terminal FLAG and His-tags, MW = 270 kDa, expressed in Baculovirus infected Sf9 cell expression system.

ALOX-15, also known as 15LOX-1, omega-6 lipoxygenase, or arachidonate 15-lipoxygenase (GenBank Accession No. NM_001140), a.a. 2- 662(end), with N-terminal FLAG-tag, MW=75 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

Human ATP citrate lyase (ACLY) (GenBank Accession No. NM_001096), full length, with N-terminal GST-tag, MW=147 kDa, expressed in baculovirus expression system.

Human AHCY (human S-adenosyl homocysteine hydrolase), GenBank Accession No. NM_000687, (a.a. 2-end) with N-terminal GST-tag, MW = 73 kDa, expressed in an E. coli expression system.

Human ALAS2 (aminolevulinate, delta-, synthase 2) (GenBank Accession No. NM_000032), (a.a. 136-553) with N-terminal His-tag, MW = 46 kDa, expressed in an E. coli expression system.

Human EZH2 (GenBank Accession No. NM_004456), a.a. 2-end with N-terminal His-GST-tag, MW= 114 kDa, expressed in a Sf9 baculovirus expression system. This protein is inactive alone, although it is functional if combined with other proteins in the complex.

Murine Pyruvate kinase M2 (PKM2), also known as PKM1/M2, isoform a (GenBank Accession No. NM_011099), a.a. 2-end, with N-terminal His-tag, MW = 58 kDa, expressed in an E. coli expression system.

Human sphingosine-1-phosphate lyase 1 (GenBank Accession No. NM_003901), a.a. 59-end, with C-terminal His-tag, MW = 57.5 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.

Human Plk1 polo box domain (GenBank Accession No. NM_005030), a.a. 367-end with N-terminal GST-tag, MW=53 kDa, expressed in an E. coli expression system.