Enzymes

Human N-terminal MBP tagged Plk3 polo box domain (GenBank Accession No.NM_004073/NP_004064), a.a. 335-646 with C-terminal His-tag, MW=80.5 kDa, expressed in an E. coli expression system.

Human MTAP (methylthioadenosine phosphorylase), GenBank Accession No. NM_002451, a.a. 2-end, with N-terminal GST-tag, MW=57 kDa, expressed in an E. coli expression system.

TurboTEV Protease contains an enhanced form of a catalytic fragment of the N1a protein of Tobacco etch virus (TEV), a cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly. TurboTEV Protease is a restriction grade protease that has a robust activity at 4°C with high specificity and great stability. It does not require any special buffer for its activity and can be used in a buffer most suitable for the target protein. TurboTEV Protease is a 52 kDa protein with both GST and His-tags so it can be easily removed by either Ni-chelating or Glutathione (GSH) resin along with the cleaved tag. In practice, 1 mg (10,000 units) of TurboTEV Protease cleaves >90% of 100 mg of a control target protein at 4°C in 16 hours. No non-specific cleavage has been observed under the same condition when TurboTEV Protease and the control target protein was mixed at 1:10 ratio.

Ultra-pure recombinant form of Serratia marcescens extracellular endonuclease. Nonspecific endonuclease, hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5'-phosphorylated oligonucleotides of 1-4 bases in length. MW = 27 kDa (homodimer). Expressed in E. coli. No detectable protease activity. TurboNuclease can be used to <br>• reduce viscosity of cell lysates<br>• reduce back pressure of column loading<br>• remove nucleic acid contamination from sample preparations<br>• reduce nucleic acid contamination of Ni column purification<br>• reduce smearing in SDS-PAGE when used with 10%SDS or Gel Loading Dye<br> • make whole cell lysates<br>• reduce or prevent clumping of concentrated cells and thawed cells<br>• replace crude DNase I in many applications

Human rhinovirus 3C protease (HRV 3C Protease) with Nterminal His-GST-tags (can be easily removed by either Ni-chelating or Glutathione (GSH) resin along with the cleaved tag.), Recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro. Cleaves between Gln and Gly. MW = 47 kDa in E. coli expression system.

Human PR domain containing 12 (PRDM12), also known as PFM9 and PR Domain Zinc Finger Protein 12 (GenBank Accession No. NM_021619), a.a. 2-243, with N-terminal GST-tag, MW=54 kDa, expressed in an E. coli cell expression system.

Human PR domain containing 14 (PRDM14), also known as PFM11 and PR Domain Zinc Finger Protein 14 (GenBank Accession No. NM_024504), a.a. 2-412, with N-terminal GST-tag, MW=72 kDa, expressed in an E. coli cell expression system.

Human JHDM1D, also known as Lysine-specific demethylase 7A (KDM7A) and KIAA1718, GenBank Accession No. NM_030647 a.a. 2-488 with an N-terminal FLAG-tag, MW=56 kDa, expressed in a baculovirus-infected Sf9 cell expression system.

Human JMJD1B, also known as lysine-specific demethylase 3B (KDM3B), JHDM2B, and KIA1082, GenBank Accession No. NM_016604/ NP_057688, a.a. 2-1761 (end) with Nterminal Avi- and FLAG-tags, MW=195 kDa, expressed in a baculovirus-infected Sf9 cell expression system.