Enzymes

Human recombinant SET domain containing lysine methyltransferase 7 (SETD7), or SET7/9 (GenBank Accession No. NM_030648), a.a. 52-266 (end) with N-terminal GST-tag, MW = 61 kDa, expressed in an E. coli expression system.

Mutant version of our regular EZH2 5-member complex (Cat. #51004), but with a Tyr-to-His mutation on a.a. 646 of the QA EZH2 protein. Complex of human EZH2 (GenBank Accession No. NM_004456), a.a. 2-end; Y646H*, with N-terminal His tag, MW= 86 kDa, human EED (NM_003797), a.a. 2-end with N-terminal Flag tag, MW= 51 kDa, human SUZ12 (NM_015355), a.a. 2-end with N-terminal His tag, MW = 87 kDa, Human AEBP2 (NM_153207), a.a. 2-end with N-terminal His tag, MW= 53 kDa, and human RbAp48 (NM_005610), a.a. 2-end with N-terminal His tag, MW = 48 kDa, co-expressed in baculovirus expression system.

Mutant version of our regular EZH2 5-member complex (Cat. #51004), but with a Tyr-to-Ser mutation on a.a. 646 of the EZH2 protein. Complex of human EZH2 (GenBank Accession No. NM_004456), a.a. 2-end, Y646S*, with N-terminal His-tag, MW= 86 kDa, human EED (NM_003797), a.a. 2-end with N-terminal FLAG-tag, MW= 51 kDa, human SUZ12 (NM_015355), a.a. 2-end with N-terminal His-tag, MW = 87 kDa, Human AEBP2 (NM_153207), a.a. 2- end with N-terminal His-tag, MW= 53 kDa, and human RbAp48 (NM_005610), a.a. 2-end with N-terminal His-tag, MW = 48 kDa, co-expressed in a baculovirus expression system.

Human SMYD2 (GenBank Accession No. NM_020197), full length with N-terminal FLAG-tag, MW= 49.7 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human SMYD3 (GenBank Accession No. NM_022743), a.a. 35-end, with N-terminal GST-tag, MW=64 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human SMYD3 (GenBank Accession No. BC031010), full length, with N-terminal FLAG-tag, MW=50 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Mutant version of our regular EZH2 5-member complex (Cat. #51004), but with a Tyr-to-Phe mutation on a.a. 646 of the EZH2 protein. Complex of human EZH2 (GenBank Accession No. NM_004456), a.a. 2-end; Y646F*, with N-terminal His tag, MW= 86 kDa, human EED (NM_003797), a.a. 2-end with N-terminal Flag tag, MW= 51 kDa, human SUZ12 (NM_015355), a.a. 2-end with N-terminal His tag, MW = 87 kDa, Human AEBP2 (NM_153207), a.a. 2-end with N-terminal His tag, MW= 53 kDa, and human RbAp48 (NM_005610), a.a. 2-end with N-terminal His tag, MW = 48 kDa, co-expressed in baculovirus expression system.

Human Metnase (SETMAR, or SET domain and mariner transposase fusion), GenBank Accession No. NM_006515, a.a. 27-684(end), with N-terminal FLAG-tag, MW=76 kDa, expressed in FreeStyle 293-F cells.

Mutant version of BPS's regular EZH2 5-member complex (Cat. # 51004), but with a Pro-to-Ser mutation on a.a. 132 of the EZH2 protein. Complex of human EZH2 (GenBank Accession No. NM_004456), (a.a. 2-end; P132S) with N-terminal His-tag, MW= 86 kDa, human EED (NM_003797) (a.a. 2-end) with N-terminal FLAG-tag, MW= 51 kDa, human SUZ12 (NM_015355) (a.a. 2-end) with N-terminal His-tag, MW = 87 kDa, Human AEBP2 (NM_153207) (a.a. 2-end) with N-terminal His-tag, MW= 53 kDa, and human RbAp48 (NM_005610) (a.a. 2-end) with N-terminal His-tag, MW = 48 kDa, co-expressed in a baculovirus expression system.