Enzymes

Human PRMT3 (GenBank Accession No. NM_005788), a.a. 2-end, with N-terminal GST-tag, MW=86 kDa, expressed in an E. coli expression system.

Complex of human PRMT5 (GenBank Accession No. NM_006109), a.a. 2-end, with N-terminal FLAG-tag, MW=73 kDa, and human MEP50 (GenBank Accession No. NM_024102), a.a. 2-end, with N-terminal His tag, MW=37.5 kDa, expressed in FreeStyle 293-F cells.

Human PRMT4 (CARM1), GenBank Accession No. NM_199141, a.a. 2-end, with N-terminal FLAG-tag, MW=66 kDa, expressed in FreeStyle 293-F cells.

Human PRMT5 (GenBank Accession No. NM_006109), a.a. 2-end, with N-terminal FLAG-tag, MW=73 kDa, and human MEP50 (GenBank Accession No. NM_024102), a.a. 2-end, with N-terminal His-tag, MW=37 kDa, coexpressed in a Baculovirus infected Sf9 cell expression system.

Human PRMT6 (GenBank Accession No. NM_018137), a.a. 2-375(end), with N-terminal His-tag, MW=43 kDa, expressed in E. coli.

Human PRMT8 (protein arginine methyltransferase 8), GenBank Accession No. NM_019854, a.a. 61-394(end) with N-terminal His-FLAG-tag, MW=40.8 kDa, expressed in Sf9 cells using a Baculovirus expression system.

Human PRMT7 (protein arginine methyltransferase 7), GenBank Accession No. NM_019023, a.a. 2-692(end) with N-terminal FLAG-tag, MW=80 kDa, expressed in Sf9 cells using a Baculovirus expression system.

Human SetDB1 (variant 2), also known as Histone-lysine Nmethyltransferase SETDB1, SET domain bifurcated 1, KMT1E, ERG-associated protein with SET domain (ESET), GenBank Accession No. NM_012432, a.a. 510- 1290(end) expressed in a Baculovirus Sf9 cell expression system. MW = 114 kDa.

Human SET and MYNDcontaining protein 4 (SMYD4), also known as KIAA1936, amino acids 2- 804(end), with N-terminal FLAG-tag GenBank Accession No. NM_052928, MW = 90 kDa. This protein is expressed in a Baculovirus infected Sf9 cell expression system.