Enzymes

Human DNMT2 (GenBank Accession No. NM_004412), (a.a. 2-391(end)), with N-terminal GST-tag, MW=71 kDa, expressed in baculovirus expression system.

Human DNMT3a (GenBank Accession No. NM_175629), full length with N-terminal GST-tag, MW=128 kDa, expressed in a baculovirus-infected Sf9 cell expression system.

Complex of human DNA methyl-transferase 3B (DNMT3B), GenBank Accession No. NM_006892, a.a. 564-853(end) with N-terminal His-tag, MW=34 kDa, and DNMT3L (GenBank Accession No. NM_013369), a.a. 160-387(end), with an N-terminal GST-tag, MW = 52 kDa, co-expressed in a baculovirus-infected Sf9 cell expression system.

Complex of active human DNA methyl-transferase 3A (DNMT3A), GenBank Accession No. NM_175629, a.a 623-912(end) with N-terminal His-tag, MW=34 kDa, and DNMT3L (GenBank Accession No. NM_013369), a.a. 160-387(end), with an N-terminal GST-tag, MW = 52 kDa, co-expressed in a baculovirus-infected Sf9 cell expression system.

Complex of human DNA methyl-transferase 3B (DNMT3B), GenBank Accession No. NM_006892, a.a. 564-853(end) with N-terminal GST-tag, MW=59 kDa, and DNMT3L (GenBank Accession No. NM_013369), a.a. 160-387(end), with an N-terminal His-tag, MW = 27 kDa, co-expressed in a baculovirus-infected Sf9 cell expression system.

Human WDR5 (WD Repeat Domain 5), also known as SWD3 or BIG-3, GenBank Accession No. NM_017588, a.a. 2-334(end), with N-terminal FLAGtag, MW=37 kDa, expressed in a baculovirus-infected Sf9 insect expression system.

Human Histone 3 (GenBank Accession No. NM_003532), a.a. 2-58 with N-terminal GST-tag, MW = 32 kDa, expressed in an E. coli expression system.

Nucleosomes prepared from mammalian HEK293 cells as a substrate for histone methyltransferase assay.

Human Histone 4 (GenBank Accession No. NM_003548), a.a. 2-58 with N-terminal GST-tag, MW = 32 kDa, expressed in an E. coli expression system.