Enzymes

Human Aurora kinase A (GenBank Accession No. NM_003600), a.a. 2-403 with N-terminal His-tag, MW=50 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human BRAF (GenBank accession # NM_004333), a.a. 432-725 with N-terminal His-tag, MW=36 kDa, and p50 (GenBank accession # NM_007065), a.a. 2-378(end) with N-terminal FLAG-tag, MW=45 kDa, coexpressed in a Baculovirus infected Sf9 cell expression system. This protein exists in a complex with Cdc37 and p50.

Human GSK3α (alpha) GenBank Accession No. BC027984), full length, with N-terminal GST-tag, MW=81.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human GSK3β GenBank Accession No. NM_002093), full length, with N-terminal GST-tag and C-terminal His-tag, MW=73 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human RAF1, also known as c-RAF (GenBank Accession No. NM_002880), a.a. 306-648, containing the mutations Y340D and Y341D, with N-terminal GST-tag, MW=65 kDa, expressed in an Baculovirus infected Sf9 cell expression system.

Human NEK2 (GenBank Accession No. NM_002497) full length with N-terminal His-tag, MW=53 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human v-raf murine sarcoma 3611 viral oncogene homolog, transcript variant 1 (A-RAF1), (GenBank Accession No. NM_001654), a.a. 282-606 (end), containing mutations YY301-302DD, with N-terminal GST-tag and C-terminal His-tag, MW=63 kDa, expressed in an Baculovirus infected Sf9 cell expression system.

Recombinant human AKT2 (GenBank Accession No. NM_001626), full length with N-terminal His-tag, MW=60.2 kDa, was expressed in Sf9 insect cells via a baculovirus expression system. The purified AKT2 is activated in vitro using PDK1 enzyme.

Recombinant human AKT3 (GenBank Accession No. NM_005465), a.a. 2-end, with N-terminal His-tag, MW= 60 kDa, expressed in a baculovirus expression system.