Enzymes

Human recombinant histone tetramer consisting of 2 molecules each of histones H2A (a.a. 1-130(end)), H2B (a.a. 1-126(end)), H3 (a.a. 1-136(end)), and H4 (a.a. 1-103(end)), expressed in an E. coli expression system. Each histone protein has an N-terminal His-tag. Histone H3 has K27C, C97A, and C111A mutations and was alkylated to produce the lysine analog, aminoethylcysteine. GenBank Accession Nos. are NM_033445, NM_003528, NM_003532, and NM_003548, respectively. Global MW = 116 kDa.

Human recombinant histone octamer consisting of 2 molecules each of histones H2A (a.a. 1-130(end)), H2B (a.a. 1-126(end)), H3 (a.a. 1-136(end)), and H4 (a.a. 1-103(end)), expressed in an E. coli expression system. Each histone protein has an N-terminal His-tag. Histone H3 has K27C, C97A, and C111A mutations and was alkylated to produce the lysine analog, dimethyl aminoethylcysteine. GenBank Accession Nos. are NM_033445, NM_003528, NM_003532, and NM_003548, respectively. Global MW = 116 kDa.

Human recombinant histone octamer consisting of 2 molecules each of histones H2A (a.a. 1-130(end)), H2B (a.a. 1-126(end)), H3 (a.a. 1-136(end)), and H4 (a.a. 1-103(end)), expressed in an E. coli expression system. Each histone protein has an N-terminal His-tag. Histone H3 has K27C, C97A, and C111A mutations and was alkylated to produce the lysine analog, trimethyl aminoethylcysteine. GenBank Accession Nos. are NM_033445, NM_003528, NM_003532, and NM_003548, respectively. Global MW = 116 kDa.

Human Histone 3 (HIST1H3E), GenBank Accession No. NM_003532, a.a. 2-136(end) with K27M mutation and N-terminal His-tag, MW = 16.2 kDa, expressed in an E. coli expression system.

PRMT5/MEP50 complex of human PRMT5 (GenBank Accession No. NM_006109), amino-acids 2-637(end), with N-terminal FLAG-Avi tag, MW=73 kDa, and human MEP50 (GenBank Accession No. NM_024102) with N-terminal His-Tag, amino-acids 2-342(end), MW=38 kDa, coexpressed in HEK293 cells and enzymatically biotin-labeled using Avi-tag™ technology.

Human SETD2, also known as SET2 and HYPB, (GenBank Accession No. NM_014159), a.a. 1418-1714, with N-terminal GST-tag, MW= 60.9 kDa, expressed in an E. coli expression system.

Human ubiquitin-like with PHD, Tudor and ring finger domains 1 (UHRF1), GenBank Accession No. NM_001048201, a.a. 2 - 793 (end) with N-terminal His-Flag tag, MW = 92 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Rat PDE1B (GenBank Accession No. NM_022710), full length with N-terminal HIS-GST-tag, MW= 89 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human PDE1A, isozyme 4 (GenBank Accession No. NM_001258313.1), full length with N-terminal GST-tag, MW= 88.3 kDa, expressed in a Baculovirus infected Sf9 cell expression system.