Enzymes

Recombinant S. solfataricus S-adenosyl homocysteine hydrolase, or AHCY, (GenBank Accession No. Z50174) a.a. 2 - 417 (end) with N-terminal His-tag, MW= 47 kDa, expressed in an E. coli expression system.

Human nitric oxide synthase 3 (NOS3), also known as endothelial NOS (eNOS), (GenBank Accession No. NM_000603), a.a. 2 - 1203 (end) with N-terminal His-FLAG-tag, MW= 135 kDa, expressed in a baculovirus infected Sf9 cell expression system.

Human activated protein C, also known as PROC, APC, or active coagulation factor XIV, (GenBank Accession No. NM_000312), a.a. 1 - 461 (end) with C-terminal His-tag, expressed in an HEK293 cell expression system. Note: Protein is secreted and converted to the active form with disulfide link (light chain a.a. 43-199, heavy chain a.a. 212-461). The signal peptide (a.a. 1-32), propeptide (a.a. 33-42), and activation peptide (a.a. 200-211) are cleaved. MW = ~60 kDa, ~42 kDa, ~20 kDa under reducing conditions.

Staphylococcus aureus sortase A Pentamutant (P94R, D160N, D165A, K190E, K196T), a.a. 26-206(end) GenBank Accession No. AF162687 with N-terminal His tag, MW = 21.7 kDa, expressed in an E. coli expression system.

Staphylococcus aureus sortase A Hexamutant (P94R, D124G, D160N, D165A, K190E, K196T), a.a 26-end, GenBank Accession No. AF162687 with N-terminal His tag, MW = 22 kDa, expressed in an E. coli expression system.

Staphylococcus aureus sortase A Heptamutant (P94R, D160N, D165A, Y187L, E189R, K190E, K196T), a.a 26-206(end) GenBank Accession No. AF162687 with N-terminal His tag, MW = 22 kDa, expressed in an E. coli expression system.

Pseudomonas putida formaldehyde dehydrogenase (FDH) a.a. 2 - 399 (end) (GenBank Accession No. D21201) with N-terminal His-tag, MW = 42.9 kDa, expressed in an E. coli expression system.

Human fucokinase (GenBank Accession No. NM_145059), a.a. 2-1084(end) with N-terminal FLAG-tag, MW = 118.6 kDa, expressed in baculovirus using an Sf9 insect cell expression system.

Human lactate dehydrogenase B (LDHB) a.a. 2 - 334 (end) (GenBank Accession No. NM_002300) with N-terminal His-tag, MW = 37.5 kDa, expressed in an E. coli expression system.