Enzymes

Human SAP145, a substrate for PRMT9, also known as Splicing Factor 3b Subunit 2 (SF3B2), (GenBank accession No. NM_006842), (a.a. 401-550) with an N-terminal GST-tag, MW= 45kDa, expressed in a Baculovirus-infected Sf9 cell expression system.

Human Jak2 with V617F mutation, GenBank Accession No. NM_004972, a.a. 513-1132 (end), with N-terminal GST tag, C-terminal Avi-Tag, expressed in a HEK293 cell expression system and enzymatically Biotin-labeled using AviTag™ technology.

Human MAT2B, also known as Methionine Adenosyltransferase 2B, MAT2, and MAT II Beta. GenBank Accession No. NM_013283, a.a. 2-334 (end) with an N-terminal His-tag, expressed in an E. coli cell expression system. MW = 39 kDa.

Human Protein Arginine Methyltransferase 9 (PRMT9), GenBank Accession No. NM_138364, a.a. 2-845 (end), with an N-terminal FLAG-tag and a C-terminal His-tag, expressed in an SF9 insect cell expression system. MW = 95 kDa.

Human PRMT5 (GenBank Accession No. NM_006109), amino-acids 2-637(end), with N-terminal FLAG-Avi tag, MW=76 kDa, expressed in HEK293 cells and enzymatically biotin-labeled using Avi-tag™ technology.

Acetyl-Coenzyme A Carboxylase (ACC) plays an important role in fatty acid metabolism so it has been proposed to be a drug target for the fatty acid-related metabolic diseases including obesity and diabetes. The Acetyl-Coenzyme A Carboxylase 2 (ACC2) assay kit is designed to measure ACC2 activity for screening and profiling applications using ADP-Glo™ reagents as a detection reagent. The Acetyl-coenzyme A carboxylase 2 (ACC2) assay kit comes in a convenient 96-well format, with enough purified recombinant ACC2 enzyme, ACC2 substrate, ATP and ACC assay buffer for 100 enzyme reactions.

Human FTO, also known as Fat mass and obesity-associated protein, ALKBH9; BMIQ14; GDFD; KIAA1752, and alpha-ketoglutarate-dependent dioxygenase, GenBank Accession No. NM_001080432, a.a 32-505 (end), with N-terminal His-Tag and expressed in a E. coli cell expression system. MW = 56 kDa.

The IDH2(R140Q) Assay Kit is designed to measure IDH2(R140Q) activity for screening and profiling applications by measuring NADPH consumption.

Acetyl-Coenzyme A Carboxylase (ACC) plays an important role in fatty acid metabolism so it has been proposed to be a drug target for the fatty acid-related metabolic diseases including obesity and diabetes. The Acetyl-Coenzyme A Carboxylase 1 (ACC1) assay kit is designed to measure ACC1 activity for screening and profiling applications using ADP-Glo™ reagents as a detection reagent