Enzymes

Human RIPK3, also known as Receptor Interacting Serine/Threonine Kinase 3 and RIP3, GenBank Accession No. NM_006871, a.a. full length, with N-terminal GST-tag, expressed in a Baculovirus-infected Sf9 cell expression system. MW = 96 kDa.

Human RIPK4, also known as ANKK2, DIK, PKK, RIP4, and ANKRD3, GenBank Accession No. BC110618, a.a. 15-426, with N-terminal GST-tag, expressed in a Baculovirus-infected Sf9 cell expression system. MW = 57 kDa.

Human RIPK5, also known as DSTYK, DustyPK, HDCMD38P, KIAA0472, and RIP5, GenBank Accession No. NM_015375, a.a. full length, with N-terminal GST-tag, expressed in a Baculovirus-infected Sf9 cell expression system. MW = 140 kDa.

Human cathepsin B (GenBank Accession No. NM_001908), CD33 signal peptide (a.a. 1-16) + CathepsinB (a.a. 18-339), with C-terminal His-tag, MW=43 kDa, expressed in FreeStyle 293-F cells.

Human cathepsin F (GenBank Accession No. NM_003793), CD33 signal peptide(a.a. 1-16) + cathepsin F (a.a. 20-end), with C-terminal His-tag, MW = 53 kDa (more than 58kDa after post-modification), expressed in FreeStyle 293-F cells.

Human cathepsin L (GenBank Accession No. NM_001912), a.a. 18-333, with C-terminal His-tag, MW=36 kDa, expressed in FreeStyle 293-F cells.

Human cathepsin S (GenBank Accession No. NM_004079.3), CD33 signal peptide(a.a. 1-16) + cathepsin S (a.a. 17-331), with C-terminal His-tag, MW=37 kDa, expressed in FreeStyle 293-F cells.

Human cathepsin V (GenBank Accession No. NM_001333.2), CD33 signal peptide(a.a. 1-16) + cathepsin V (a.a. 18-334), with C-terminal His-tag, MW=37 kDa, expressed in FreeStyle 293-F cells.

Fusion protein corresponding to the serine protease NS3 (a.a. 3-181) and cofactor NS4A (a.a. 21-32) from Hepatitis C virus genotype 1a (HCV1a), GenBank Accession No. NC_004102, with N-terminal FLAG-His tag, MW = 22.4 kDa, expressed in an E. coli expression system.