Enzymes

TNK1 (Tyrosine Kinase Non Receptor 1), also known as CD38 Negative Kinase 1 (GenBank Accession No. NM_003985), a.a. 106-390, with N-terminal GST-tag and Thrombin Cleavage Site, MW = 57 kDa, expressed in Sf9 cells via a baculovirus expression system.

Human DPP3 (GenBank Accession No. NM_130443), full length with N-terminal GST-tag, MW=109 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human DPP4 (GenBank Accession No. NM_001935), a.a. 29-end with C-terminal His-tag, MW=89.1 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human DPP7 (GenBank Accession No. NM_013379), a.a. 26-end with C-terminal His-tag, MW=52.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human DPP8 (Dipeptidylpeptidase 8), GenBank Accession No. NM_197960, full length with N-terminal GST-tag, MW=129.3 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Recombinant human DPP9 (dipeptidyl peptidase 9), isoform 1 (short form), encompassing amino acids 1-863(end). This construct contains an N-terminal GST-tag. The recombinant protein was affinity purified, expressed in a Baculovirus infected Sf9 cell expression system.

Human FAP (GenBank Accession No. NM_004460), (a.a. 29-end) with C-terminal His-tag, MW=85.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Fusion protein corresponding to the serine protease NS3 (a.a. 3-181) and cofactor NS4A (a.a. 21-32) from Hepatitis C virus genotype 1a (HCV1a), GenBank Accession No. NC_004102, with Arg-to- Lys mutation on a.a. 155, and N-terminal FLAG-His tag, MW = 22.4 kDa, expressed in an E. coli expression system.

Fusion protein corresponding to the serine protease NS3 (a.a. 3-181) and cofactor NS4A (a.a. 21-32) from Hepatitis C virus genotype 1a (HCV1a), GenBank Accession No. NC_004102, with Asp-to- Glu mutation on a.a. 168, and N-terminal FLAG-His tag, MW = 22.4 kDa, expressed in an E. coli expression system.