Enzymes

Fusion protein corresponding to the serine protease NS3 (a.a. 3-181) and cofactor NS4A (a.a. 21-32) from Hepatitis C virus genotype 1b (GenBank Accession No. AF054247) with Arg-to-Gln mutation on a.a. 155, an N-terminal FLAG-His tag, and a 4 a.a. linker, MW = 22.3 kDa, expressed in an E. coli expression system.

Human Prolyl oligopeptidase (POP)/Prolyl endopeptidase (PREP) (GenBank Accession No. NM_002726) full length with N-terminal His-tag, MW = 81.6 kDa, expressed in an E. coli expression system.

Hepatitis C virus (HCV) genotype 1b, also known as HCV1b, is a fusion protein of a.a. 21-32 of NS4A, linker (3 a.a.) and a.a. 31-181 of NS3 (GenBank Accession No. AF054247) with N-terminal FLAG-His-tag, MW = 22.3 kDa, expressed in an E. coli expression system.

Hepatitis C virus (HCV) genotype 2a, also known as HCV2a, is a fusion protein of a.a. 21-32 of NS4A, linker (4 a.a.) and a.a. 3-181 of NS3 (GenBank Accession No. FJ393024) with N-terminal FLAG-His-tag, MW = 22.3 kDa, expressed in an E. coli expression system.

Fusion protein corresponding to the serine protease NS3 (a.a. 3-181) and cofactor NS4A (a.a. 21-32) from Hepatitis C virus genotype 1b (GenBank Accession No. AF054247) with Arg-to-Lys mutation on a.a. 155, an N-terminal FLAG-His tag, and a 4 a.a. linker, MW = 22.3 kDa, expressed in an E. coli expression system.

Hepatitis C virus (HCV) genotype 1b, also known as HCV1b, is a fusion protein of a.a. 21-32 of NS4A, linker (4 a.a.) and a.a. 3-181 of NS3 (GenBank Accession No. AF054247) with Asp-to-Val mutation on a.a. 168, and N-terminal FLAG-His-tag, MW = 22.3 kDa, expressed in an E. coli expression system.

The Human Thrombin was activated from homogeneous human prothrombin by activation with Factor Xa, Factor Va, and phospholipid. These activating enzymes were removed after activation. Complete activation was observed on SDS-PAGE. MW = 37 kDa.

The Human Factor Xa was activated from Human Factor X by activation with Russell's Viper Venom. This RVV-X was removed after activation. Complete activation is observed on SDS-PAGE. MW = 46 kDa.

Human Caspase 9 (CASP9), GenBank Accession No. NM_001229, a.a. 1 - 416 (end), with C-terminal His-tag, MW=35 &10 kDa, expressed in an E. coli cell expression system. Procaspase-9 is autocleaved between the prodomain and large protease subunit (p35). The cleavage product contains both the large (p35) and small (p10 [His-tag]) protease subunits.