Enzymes

Human SMURF2 (GenBank Accession No. NM_022739), a.a. 130-end, with N-terminal FLAG-tag, MW= 72 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human Nedd4 (GenBank Accession No. NM_006154), a.a. 150-end, with N-terminal FLAG-tag, MW= 86 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human WWP1 (GenBank Accession No. NM_007013), a.a. 2-end, with N-terminal FLAG-tag, MW=106 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human deubiquitinating enzyme ovarian tumor domain-containing 6B, or OTUD6B (GenBank Accession No. NM_016023), a.a. 2-323 (end) with N-terminal His-FLAG-tag, MW=39 kDa, expressed in E. coli cells.

Human A20, also known as tumor necrosis factor-? induced protein 3 (TNFAIP3) or OTU domain-containing protein 7C (OTUD7C), GenBank Accession No. NM_006290, amino-acids 2-790(end) with an N-terminal FLAG-tag and C-terminal His-tag, MW=91 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

Complex of human cullin-3 (CUL3) with human RING box protein 1 (Rbx1), also known as E3 ubiquitin-protein ligase RBX1. CUL3 (a.a. 2-768(end) with N-terminal GSTtag, 117 kDa) is co-expressed with Rbx1 (a.a. 1-108(end), 12 kDa) in a baculovirus infected Sf9 cell expression system. GenBank Accession Nos. are NM_003590 (CUL3) and NM_014248 (Rbx1).

Human Potassium Channel Tetramerization Domain Containing 13 (KCTD13) protein, also known as BTB/POZ domain-containing adapter for CUL3- mediated RhoA degradation protein 1 (BACURD1), GenBank Accession No. NM_178863, a.a. 2-329(end) with N-terminal GST-tag, MW = 63 kDa, expressed in a baculovirus-infected Sf9 cell expression system.

Human E3 UFM1-protein ligase 1 (UFL1), also known as LZAP-binding protein, GenBank Accession No. NM_015323, a.a. 2-794 with N-terminal GST-tag, MW = 116 kDa, expressed in a baculovirus-infected Sf9 cell expression system.

Human Casitas B-lineage lymphoma proto-oncogene b (CBL-B), also known as E3 ubiquitin-protein ligase CBL-B, RING finger protein 56, and SH3-binding protein CBL-B, GenBank Accession No. NM_170662, a.a. 39 - 426 with N-terminal His-tag and N-terminal Avi-tag, expressed in an E. coli expression system. MW=48 kDa.