Enzymes

Mouse FAP, GenBank Accession No. NM_007986, amino acids 26-762 (end), with C-terminal His-tag, MW=88 kDa, expressed in HEK293 expression system.

Dog PDE3A, also known as Phosphodiesterase 3A, CGMP-Inhibited 3',5'-Cyclic Phosphodiesterase A, and CGI-PDE A, GenBank Accession No. XP_005637137, a.a. 343-998 (end), with C-terminal FLAG-tag, expressed in a HEK293 expression system, MW = 75 KDa.

Human CUL2, also known as Cullin-2 and CUL-2 with N-terminal FLAG-tag, a.a 2-745 (end), GenBank Accession No. NM_003591, expressed in a HEK293 expression system. MW = 88 kDa.

MLL3/WARD complex of human MLL3 (GenBank Accession No. NM_170606) a.a. 4689-4911(end) with N-terminal His-tag and MW= 27 kDa, human WDR5 (GenBank Accession No. NM_017588), a.a. 2-334(end ) with N-terminal His-tag and MW= 37 kDa, human Ash2L (GenBank Accession No. NM_001105214), a.a. 2-534(end) with N-terminal His-tag and MW= 61 kDa, human RbBP5 (GenBank Accession No. NM_005057), a.a. 2-538(end) with N-terminal His-tag and MW= 60 kDa, and human DPY30 (GenBank Accession No. NM_032574) a.a. 2-99(end) with N-terminal His-tag and MW=12 kDa individually expressed in an E. coli expression system.

Mouse RIPK1, also known as RCK and MAP3K19, GenBank Accession No. NM_009068, a.a. 1-327, with N-terminal GST-tag, expressed in a Baculovirus-infected Sf9 cell expression system. MW = 64 kDa.

Human recombinant SHP-1, also known as tyrosine-protein phosphatase non-receptor type 6 (PTPN6), protein tyrosine phosphatase 1 C (PTP- 1C), and SH-PTP1, Genbank accession number NM_002831, a.a. 221-523 with N-terminal His-tag. MW=36 kDa, expressed in an E. coli expression system.

Human Large Tumor Suppressor Kinase 2 (LATS2) protein, also known as KPM and LATS, GenBank Accession No. NM_014572, a.a 480-1088(end), with an N-terminal GST-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW = 97 kDa.

Human FGFR2, also known as Fibroblast growth factor receptor 2, Keratinocyte growth factor receptor, BBDS, BEK, BFR-1 and CD332, GenBank Accession No. NM_000141, a.a. 400-821(end), with deletion at 429-430, with C-terminal His-tag, expressed in an Sf9 infected baculovirus expression system. MW = 52 kDa. This protein was isolated from a co-expression with PTP1B. Percentage of phosphorylation ≤ 10%.

Human MALT1 also known as IMD12, MLT, MLT1, PCASP1, and MALT1 paracaspase, GenBank Accession No. NM_006785, a.a. 2-824 (end), and expressed in HEK293 cells. MW = 93 kDa.