Enzymes

Human Tyrosine Kinase 2 (TYK2), also known as JTK1, IMD35, and Non-Receptor Tyrosine-Protein Kinase TYK2, Genbank Accession No. NM_003331, a.a. 575-869 with N-terminal FLAG-tag, expressed in Sf9 cells via a baculovirus expression system. MW = 34 kDa.

CDC7/DBF4 complex of human CDC7, also known as HsCDC7 and Hsk 1, GenBank Accession No. NM_003503 a.a. 1-574(end) with N-terminal GST-tag and MW= 94 kDa, and human DBF4, also known as ASK and CHIF, GenBank Accession No. NM_006716, a.a. 1-674(end) with N-terminal GST-tag and MW= 125 kDa, co-expressed expressed in an Sf9 infected baculovirus expression system.

Human matrix metallopeptidase-8 (MMP8), also known as HNC, CLG1, MMP-8 and PMNL-CL, GenBank Accession No. NM_002424, a.a. 21-467(end) with C-terminal His-tag, expressed in a HEK293 cell expression system. MW=52 kDa. This protein runs at a higher molecular weight due to glycosylation.

Complex of CDK19, also known as CDK11 and CDC2L6, and Cyclin C proteins. The two proteins were co-expressed and affinity purified. Recombinant human CDK19 (cyclin dependent kinase 19), full length encompassing amino acids 1-502 (end). Recombinant human Cyclin C (also known as CCNC), full length encompassing amino acids 1-283 (end). Both constructs contain an N-terminal GST-tag.

Complex of CDK11A and Cyclin D3 proteins. Recombinant human CDK11A (Cyclin-dependent kinase 11A), encompassing amino acids 361-780 (end). Recombinant human Cyclin D3 (also known as CCND3), full length encompassing amino acids 1-292 (end). Both constructs contain an N-terminal GST-tag. The two proteins were co-expressed in a Baculovirus infected Sf9 cell expression system and affinity purified.

CDK14/Cyclin Y complex of human CD14, also known as PCTK1, CDK16, and PCTAIRE1 GenBank Accession No. NM_012395, a.a. 1-469(end) with N-terminal GST-tag, MW=72 kDa, and human Cyclin Y, also known as CCNY, CBCP1, CCNX, and CFP1, GenBank Accession No. NM_145012, a.a. 2-341(end) with N-terminal GST-tag. MW= 64 kDa. Co-expressed in a Baculovirus infected Sf9 cell expression system.

CDK16/Cyclin Y complex of human CDK16, also known as PCTK1 and PCTAIRE1 GenBank Accession No. NM_006201, a.a. 107-496(end) with N-terminal GST-tag, MW=66 kDa, and human Cyclin Y, also known as CCNY, CBCP1, CCNX, and CFP1, GenBank Accession No. NM_145012, a.a. 2-341(end) with N-terminal GST-tag. MW= 64 kDa. Co-expressed in a Baculovirus infected Sf9 cell expression system.

CDK17/Cyclin Y complex of human CDK17, also known as PCTK2, CDK17, PCTAIRE2, GenBank Accession No. NM_002595, a.a. 1-523(end) with N-terminal GST-tag, MW=88 kDa, and human Cyclin Y, also known as CCNY, CBCP1, CCNX, and CFP1, GenBank Accession No. NM_145012, a.a. 2-341(end) with N-terminal GST-tag. MW= 64 kDa. Co-expressed in a Baculovirus infected Sf9 cell expression system.

CDK18/Cyclin Y complex of human CD18, also known as PCTK3, PCTAIRE3, and CDK18, GenBank Accession No. BC011526, a.a. 1-523(end) with N-terminal GST-tag, MW=76 kDa, and human Cyclin Y, also known as CCNY, CBCP1, CCNX, and CFP1, GenBank Accession No. NM_145012, a.a. 2-341(end) with N-terminal GST-tag. MW= 64 kDa. Co-expressed in a Baculovirus infected Sf9 cell expression system.