Enzymes

Recombinant mouse CDK20 (cyclin dependent kinase 20), full length encompassing amino acids 2-346 (end). This construct contains an N-terminal GST-tag followed by thrombin cleavage site. The recombinant protein was affinity purified.

Human Fibroblast growth factor receptor 3 (FGFR3), also known as JTK4, CD333, CEK2 and ACH, GenBank Accession No. NM_00142, a.a. 447-761, with N-terminal His-Avi-tag, expressed in an Sf9 infected baculovirus expression system and enzymatically biotinylated using Avi-tag™ technology. Biotinylation is confirmed to be ≥90%. MW = 39 kDa. This protein was isolated from a co-expression with PTP1B. Percentage of phosphorylation ≤5%.

Human MAP4K3, also known as GLK, MAPKKKK3, MEKKK3 and RAB8IPL1, GenBank Accession No. NM_003618, a.a. 1-380, with N-terminal GST-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW= 70 kDa.

Human transforming growth factor beta receptor 2 (TGFβR2), also known as TGFBR2, TGFR-2, and TGF-Beta Receptor Type 2, GenBank Accession No. NM_003242, a.a. 23-166 fused at the C-terminus of the Fc portion of human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system. MW = 45 kDa. This protein runs at a higher molecular weight due to glycosylation.

Human MET, also known as Proto-Oncogene c-Met, HGF Receptor (HGFR), AUTS9 and SF Receptor, GenBank Accession No. NM_000245, a.a. 956-1390(end) with deletion a.a. 963-1009 (exon 14) and N-terminal GST-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW=71 kDa.

Human Transforming growth factor beta receptor I (TGFβR1), also known as activin A receptor type II-like kinase, AAT5 and LDS1, GenBank Accession No. NM_004612.4, a.a 34-126, fused at the C-terminus of the Fc portion of human IgG1, with C-terminal Avi-tag™, expressed in a HEK293 cell expression system. MW=39 kDa. This protein runs at a higher molecular weight due to glycosylation.

Human Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1B (DYRK1B), also known as Minibrain-Related Kinase (MIRK), GenBank Accession No. NM_004714, a.a. 2-629(end), with N-terminal GST-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW= 95 kDa.

Human Fibroblast Growth Factor Receptor 3 (FGFR3), also known as JTK4, and Hydroxyaryl-Protein Kinase with V555F mutation, GenBank Accession No. NM_000142, a.a. 447-761, with N-terminal His-tag, co-expressed with PTP1B for dephosphorylation in an Sf9 cell expression system, phosphorylation level <5%. MW = 37 kDa.

Mouse transforming growth factor beta receptor 2 (TGFβR2), also known as TGFBR2, TGFR-2, and TGF-Beta Receptor Type 2, GenBank Accession No. NM_009371, a.a. 24-184 fused at the C-terminus of the Fc portion of human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system, MW = 47 kDa. This protein runs at a higher molecular weight due to glycosylation.