Enzymes

Transglutaminase from K. albica, Genbank accession # AHI00814.1, a.a. 29-262(end) with a C-terminal His-tag, expressed in E. coli. MW = 28 kDa

Complex of Human JAK2, also known as Janus Kinase 2, with V617F, Y1007F and Y1008F mutations, GenBank Accession No. NM_004972, a.a. 36-1132(end), with N-terminal FLAG-tag, MW=128 kDa, and human EPOR, also known as Erythropoietin receptor, GenBank Accession No. NM_000121, a.a. 273-338 with N-terminal His-tag, MW=9 kDa. The proteins were expressed as single fusion protein with a (GSSG)₈ linker in a Baculovirus infected Sf9 cell expression system.

Complex of Human EPOR, also known as Erythropoietin receptor, GenBank Accession No. NM_000121, a.a. 273-338 with N-terminal His-tag, MW=9 kDa, and JAK2, also known as Janus Kinase 2, with V617F, Y1007F and Y1008F mutations, GenBank Accession No. NM_004972, a.a. 36-1132(end), with N-terminal FLAG-tag, MW=128 kDa. The proteins were expressed as single fusion protein with a (GSSG)₆ linker in a Baculovirus infected Sf9 cell expression system.

Recombinant human ALK2 (Activin receptor-like kinase 2) encompassing amino acids 147-509 (end) and containing the clinically relevant mutation R206H. In this construct the N-terminal end of ALK2 is fused with GST. A short peptide linker placed between GST and PARP7 includes a thrombin site to facilitate removal of the GST portion. The recombinant protein was affinity purified.

Human Fibroblast Growth Factor Receptor 3 (FGFR3), also known as JTK4, and Hydroxyaryl-Protein Kinase with K650E mutation, GenBank Accession No. NM_000142, a.a. 447-761, with N-terminal His-tag, co-expressed with PTP1B for dephosphorylation in an Sf9 cell expression system, phosphorylation level <5%.<br />MW = 37 kDa.

Human Fibroblast Growth Factor Receptor 3 (FGFR3), also known as JTK4, and Hydroxyaryl-Protein Kinase with K650M mutation, GenBank Accession No. NM_000142, a.a. 447-761, with N-terminal His-tag, co-expressed with PTP1B for dephosphorylation in an Sf9 cell expression system, phosphorylation level <5%.<br />MW = 37 kDa.

Human glycogen synthase kinase 3 alpha (GSK3α), also known as GSK3a and GSK3alpha, GenBank Accession No. NM_019884, a.a. 2-483(end), with N-terminal GST-tag and C-terminal His-tag, expressed in a Baculovirus infected Sf9 cell expression system, MW=78 kDa.

Dephosphorylated recombinant FGFR3 (Fibroblast Growth Factor Receptor 3) encompassing a.a. 397-806, with an N540K mutation and an N-terminal His-Tag. This protein was co-expressed with PTP1B for dephosphorylation. Phosphorylation level <5%.

Dephosphorylated recombinant FGFR3 (Fibroblast Growth Factor Receptor 3) encompassing a.a. 397-806, with an N-terminal His-Tag. This protein was co-expressed with PTP1B for dephosphorylation. Phosphorylation level <5%.