Enzymes

Human Serine/threonine-protein kinase 19 (STK19), also known as G11 and RP1, GenBank Accession No. NM_004197, a.a. 1-368(full length), with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system. MW=70 kDa.

Dephosphorylated recombinant FGFR3 (Fibroblast Growth Factor Receptor 3) encompassing amino acids 397-806. This protein contains an N-terminal His-Tag and has been biotinylated using an N-terminal Avi-Tag™. The recombinant protein is purified to ≥90%

Dephosphorylated recombinant FGFR3 (Fibroblast Growth Factor Receptor 3) encompassing amino acids 397-806. This protein contains the pathogenic mutation N540K in the tyrosine kinase domain. It also contains an N-terminal His-Tag. This protein was co-expressed with PTP1B for dephosphorylation. Phosphorylation level confirmed to be <5%. The protein is biotinylated using an N-terminal Avi-Tag™.

Recombinant human PDK3 (Pyruvate dehydrogenase kinase isoform 3), encompassing amino acids 2-406 (end). In this construct the N-terminal end of PDE7B is fused with GST. A short peptide linker placed between GST and PARP3 includes a thrombin site to facilitate removal of the GST portion. The recombinant protein was affinity purified.

Recombinant human DGKA (diacylglycerol kinase alpha, variant 3), encompassing amino acids 2-735 (end). This construct contains a N-terminal His-tag (6xHis) followed by an Avi-Tag™. The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified.

Recombinant human WNK1 (WNK lysine deficient protein kinase 1), encompassing amino acids (181-507). This construct contains an N-terminal GST-tag. The recombinant protein was affinity purified.

Recombinant human WNK2 (WNK lysine deficient protein kinase 2), encompassing amino acids (1-489). This construct contains and N-terminal GST-tag. The recombinant protein was affinity purified.

Recombinant human WNKS3 (WNK lysine deficient protein kinase 3), encompassing amino acids (1-571). This construct contains an N-terminal GST-tag. The recombinant protein was affinity purified.

Recombinant human WNKS4 (WNK lysine deficient protein kinase 4), encompassing amino acids (1-441). This construct contains an N-terminal GST-tag. The recombinant protein was affinity purified.