Enzymes

Mouse transforming growth factor beta receptor 2 (TGFβR2), also known as TGFBR2, TGFR-2, and TGF-Beta Receptor Type 2, GenBank Accession No. NM_009371, a.a. 24-184 fused at the C-terminus of the Fc portion of human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system and enzymatically biotinylated using Avi-tag™ technology. Biotinylation is confirmed to be ≥90%, MW = 47 kDa. This protein runs at a higher molecular weight due to glycosylation.

Mouse transforming growth factor beta receptor 2 (TGFβR2), also known as TGFBR2, TGFR-2, and TGF-Beta Receptor Type 2, GenBank Accession No. NM_009371, a.a. 24-184 fused at the C-terminus of the Fc portion of human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system and enzymatically biotinylated using Avi-tag™ technology. Biotinylation is confirmed to be ≥90%, MW = 47 kDa. This protein runs at a higher molecular weight due to glycosylation.

Human Fibroblast growth factor receptor 1 (FGFR1), also known as BFGFR, CD331, CEK and FLT2, GenBank Accession No. NM_0023110.3, a.a. 399-822(end), with C-terminal Avi-His-tag, co-expressed with PTP1B for dephosphorylation in an Sf9 cell expression system. Percentage of phosphorylation ≤5% and enzymatically biotinylated using Avi-tag™ technology. Biotinylation is confirmed to be ≥90%. MW=51 kDa.

Human Fibroblast growth factor receptor 1 (FGFR1), also known as BFGFR, CD331, CEK and FLT2, GenBank Accession No. NM_0023110.3, a.a. 399-822(end), with C-terminal Avi-His-tag, co-expressed with PTP1B for dephosphorylation in an Sf9 cell expression system. Percentage of phosphorylation ≤5% and enzymatically biotinylated using Avi-tag™ technology. Biotinylation is confirmed to be ≥90%. MW=51 kDa.

Human Caseinolytic Mitochondrial Matrix Peptidase Proteolytic Subunit (CLPP), also known as DFNB81 and PRLTS3, GenBank Accession No. NM_006012.4, a.a. 57-277(end), with N-terminal His-tag, expressed in an E.coli cell expression system. MW=25 kDa.

Human Caseinolytic Mitochondrial Matrix Peptidase Proteolytic Subunit (CLPP), also known as DFNB81 and PRLTS3, GenBank Accession No. NM_006012.4, a.a. 57-277(end), with N-terminal His-tag, expressed in an E.coli cell expression system. MW=25 kDa.

Human Protein Tyrosine Phosphatase Receptor Type Q (PTPRQ, Genbank NM_001145026), a.a. 2003-2259 with N-terminal His-tag, expressed in E. coli. MW = 31 kDa.

Human Protein Tyrosine Phosphatase Receptor Type Q (PTPRQ, Genbank NM_001145026), a.a. 2003-2259 with N-terminal His-tag, expressed in E. coli. MW = 31 kDa.

Recombinant human GFRAL (GDNF family receptor alpha like) encompassing amino acids 19-351 (end). This protein is fused in C-terminal with the Fc fragment of human IgG1 followed by a C-terminal Avi-Tag™. The recombinant protein was affinity purified.