Assays & Kits

The WDR9 TR-FRET Assay Kit is designed to measure the inhibition of the binding of WDR9, also known as BRWD1, to its substrate in a homogeneous 384 reaction format. This FRETbased assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, WDR9, substrate, and an inhibitor is incubated for 2 hours. Then, the fluorescence intensity is measured using a fluorescence reader.

The BPTF TR-FRET Assay Kit is designed to measure the inhibition of the binding of BPTF, also known as FALZ, to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BPTF, substrate, and an inhibitor is incubated for 2 hours. Then, the fluorescence intensity is measured using a fluorescence reader.

Poly-(Glu,Tyr 4:1) peptide is reconstituted in BPS proprietary buffer. 0.2 mg/ml at final concentration is enough for a majority of PTKs.

The epidermal growth factor receptor (EGFR; ErbB-1; HER1) is the cell-surface receptor for members of the epidermal growth factor family. Overexpression and/or hyperactivation of EGFR kinase is associated with several human cancers such as lung, glioblastoma, and epithelian tumors of the neck and head, leading to the development of anticancer therapeutics targeting EGFR. The EGFR Kinase Assay Kit is designed to measure EGFR Kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The EGFR Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant EGFR enzyme, EGFR substrate, ATP and kinase assay buffer for 100 enzyme reactions.

The EGFR(T790M/L858) Kinase Assay Kit is designed to measure EGFR (T790M/L858R) Kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The EGFR (T790M/L858R) Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant EGFR(T790M/L858R) enzyme, EGFR substrate, ATP and kinase assay buffer for 100 enzyme reactions.

The epidermal growth factor receptor (EGFR; ErbB-1; HER1) is the cell-surface receptor for members of the epidermal growth factor family. Overexpression and/or hyperactivation of EGFR kinase is associated with several human cancers such as lung, glioblastoma, and epithelian tumors of the neck and head, leading to the development of anticancer therapeutics targeting EGFR. EGFR(T790M) mutation is found as a second-site mutation in ~50% of EGFR-mutated lung tumors. The mutant was shown to be resistant to most tyrosine kinase inhibitor medicines including Erlotinib and Gefitinib. The EGFR(T790M) Kinase Assay Kit is designed to measure EGFR (T790M) Kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The EGFR (T790M) Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant EGFR(T790M) enzyme, EGFR substrate, ATP and kinase assay buffer for 100 enzyme reactions.

The epidermal growth factor receptor (EGFR; ErbB-1; HER1) is the cell-surface receptor for members of the epidermal growth factor family. Overexpression and/or hyperactivation of EGFR kinase is associated with several human cancers such as lung, glioblastoma, and epithelian tumors of the neck and head, leading to the development of anticancer therapeutics targeting EGFR. EGFR(L858R) mutation is found in ~40% of EGFR-mutated lung tumors. The EGFR(L858R) Kinase Assay Kit is designed to measure EGFR (L858R) Kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

Vascular endothelial growth factor receptor 2 (VEGFR2), also called Kinase insert Domain receptor (KDR), is a tyrosine kinase (TK) receptor for VEGFs that plays a central role in tumor angiogenesis; therefore the inhibition of VEGFR2 is a promising therapeutic strategy for inhibiting angiogenesis and tumor growth. The VEGFR2 Kinase Assay Kit is designed to measure VEGFR2 kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

The epidermal growth factor receptor (EGFR; ErbB-1; HER1) is the cellsurface receptor for members of the epidermal growth factor family. Overexpression and/or hyperactivation of EGFR kinase is associated with several human cancers such as lung, glioblastoma, and epithelian tumors of the neck and head, leading to the development of anticancer therapeutics targeting EGFR. T790M, L858R and C797S mutations in EGFR cause resistance to known EGFR inhibitors. The EGFR(T790M/C797S/L858R) Kinase Assay Kit is designed to identify mutant-selective EGFR kinase inhibitors and to measure EGFR (T790M/C797S/L858R) kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.