PCR & RT Kits

5 ml lysis buffer, 200 µl DNAse I, one-step SYBR green RT-qPCR Kit

The 2 x Taq PCR Mix with MgCl2 is a premixed solution containing everything needed for a successful PCR reaction except specific primers and DNA templates. The mix includes high-quality recombinant Taq DNA polymerase, nucleotides, and magnesium in a PCR reaction buffer. For the reaction set-up add the PCR Mix (10 or 25 uL) to the primers, template, and water for the total reaction volume of 20 or 50 uL.

HotTaq PCR mix is a prepared solution containing everything needed for a successful PCR reaction except specific primers and DNA templates. The mix includes high quality recombinant Taq DNA polymerase, and nucleotides in a specifically optimized buffer formulation. For reaction set-up add the PCR Mix (25 ul) to the primers, template and water (is provided in the set; total volume of 50 ul). PCR Mix is provided with MgCl₂ in the solution.

2 x RedTaq PCR Mix with MgCl2 is a prepared solution containing everything needed for a successful PCR reaction except specific primers and DNA templates. The mix includes high quality recombinant LongTaq DNA polymerase, and nucleotides in a specifically optimized buffer formulation. For reaction set-up add the PCR Mix (25 ul) to the primers, template and water (is provided in the set; total volume of 50 ul). PCR Mix is provided with MgCl₂ in the solution.

Manufactured and tested for use as a component in PCR reactions. Validated as well for use with highly sensitive PCR reactions including those with eubacterial primer sets. Stored in convenient 2 mL tubes to avoid unnecessary melting and freezing of the whole water supply.

25 mM MgCl₂ can be used for optimization of magnesium ion concentration in PCR reaction.