Cell Line

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant clonal HEK293 stable cell line constitutively expressing full length human Transferrin Receptor Protein-1 (TFRC and TFR1, also known as human CD71 protein, GenBank accession #NM_001128148).  The surface expression of TFRC was confirmed by flow cytometry.

Please note this product may be subject to fees, we invite you to contact your local office. The GAS reporter (Luc)-HeLa cell line is designed to monitor the activity of interferon gamma-induced signal transduction pathways in cultured cells by measuring activated STAT1 homodimers. It contains a firefly luciferase gene driven by three copies of the interferon gamma-activated sites (GAS) located upstream of the minimal TATA promoter. IFNγ first binds to a heterodimeric receptor consisting of two chains, IFNGR1 and IFNGR2, causing its dimerization and the activation of specific Janus family kinases (JAK1 and JAK2). Two STAT1 molecules associate with this ligand-activated receptor complex and are activated by phosphorylation to form active homodimer. The active STAT1 homodimers translocate to the nucleus where they bind interferon gamma-activated sites (GAS) in the promoter of IFNγ inducible genes, including luciferase reporter gene.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant stable clonal CHO cell line constitutively expressing full length human LIGHT protein, also known as TNFSF14, (Genbank #NM_003807). Surface expression is confirmed by flow cytometry.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant CHO-K1 stably expressing human HVEM (TNF receptor superfamily member 14; TNFRSF14; TR2; ATAR; HVEA; HVEM; CD270; LIGHTR; GenBank Accession #NM_003820).

Please note this product may be subject to fees, we invite you to contact your local office. The NFAT Reporter - Hek293 cell line contains a firefly luciferase gene under the control of NFAT response element stably integrated into Hek293 cells. This cell line is validated for the response to the stimulation of phorbol 12-myristate 13-acetate (PMA) with ionomycin.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant clonal stable Jurkat T cell line expressing firefly luciferase gene under the control of 4 copies of NF-κB response elements with constitutive expression of human HVEM (Herpes Virus Entry Mediator, Tumor Necrosis Factor Receptor Superfamily Member 14, TNFRSF14, CD270, GenBank Accession No. NM_003820). Following activation by human HVEM ligand LIGHT, NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase reporter gene. Materials Required but Not Supplied: Assay Medium: Thaw Medium 2 (BPS Cat. #60184) LIGHT, His-Tag (Human) (BPS Cat. #71266 LIGHT-CHO Recombinant Cell Line (BPS Cat. # 79262) 96-well tissue culture-treated white clear-bottom assay plate One-Step luciferase assay system (BPS bioscience # 60690) or other luciferase reagents for measuring firefly luciferase activity Luminometer

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant HEK293 stably expressing human HVEM (Herpesvirus entry mediator; CD270; TNFRSF14; ATAR) GenBank Accession # NM_003820).

Please note this product may be subject to fees, we invite you to contact your local office. The RARα Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of retinoic acid receptor alpha (RARα). The pack contains the RARα Reporter (Luc)-HEK293 Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARα (GenBank Accession No. NM_000964). This cell line is functionally validated for the response to the stimulation of all-trans retinoic acid, and the expression of RARα is confirmed by Western blotting. Additionally, the pack includes cell culture medium (Thaw Medium 6) that has been optimized for use with HEK293 cells. Thaw Medium 6 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection

Please note this product may be subject to fees, we invite you to contact your local office. The RARβ Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of retinoic acid receptor beta (RARβ). The pack contains the RARβ Reporter (Luc)-HEK293 Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARα (GenBank Accession No. P10826-2). This cell line is functionally validated for the response to the stimulation of all-trans retinoic acid, and the expression of RARβ is confirmed by Western blotting. Additionally, the pack includes cell culture medium (Thaw Medium 6) that has been optimized for use with HEK293 cells. Thaw Medium 6 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection o