Lipids

Sphinganine-biotin is a biotin conjugated sphinganine that can be used for binding streptavidin coated surfaces.Sphinganine (or Dihydrosphingosine) is an intermediate in the sphingolipid biosynthetic pathway. It is formed by the reduction of 3-Ketodihydrosphingosine Reductase (KDHR). Sphinganine is then acylated by Dihydroceramide synthase but can also act as a substrate for sphingosine kinases. Sphinganine can also inhibit Protein kinase C. Sphinganine-biotin is labeled at the terminal position and can be used for binding streptavidin coated surfaces.Bulk quantities of this product are available. Please email echelon@echelon-inc.com for more information.

Sphinganine-fluorescein is a fluorophore conjugated sphinganine that can be used for fluorescent analysis of lipid synthesis.Sphinganine (or Dihydrosphingosine) is an intermediate in the sphingolipid biosynthetic pathway. It is formed by the reduction of 3-Ketodihydrosphingosine Reductase (KDHR). Sphinganine is then acylated by Dihydroceramide synthase but can also act as a substrate for sphingosine kinases. Sphinganine can also inhibit Protein kinase C. Sphinganine-fluorescein is labeled at the terminal position (ex/em = 494/520 nm).Bulk quantities of this product are available. Please email echelon@echelon-inc.com for more information.

Sphingosine 1-phosphate (S1P) is a bioactive lysophospholipid formed by phosphorylation of sphingosine by sphingosine kinases. S1P binds to five G-protein coupled receptors designated S1P1-5 leading to cell proliferation motility and has a role in regulation cell shape. In addition S1P acts as an intracellular mediator in a receptor-independent manner regulating cell survival and Ca2+ homeostasis.Solubility1 mg/mL warm acetic acid0.3 mg/mL MeOH (alternately vortex for 1 min then sonicate in a bath sonicator for 10 minutes until a clear solution is obtained. Heating the solution (50-65°C) can also help with solubilization)Bulk quantities of this product are available. Please email echelon@echelon-inc.com for more information. Powered by Bioz See more details on Bioz

Sphingosine 1-phosphate (S1P) is a bioactive lysophospholipid formed by phosphorylation of sphingosine by sphingosine kinases. S1P binds to five G-protein coupled receptors designated S1P1-5 leading to cell proliferation motility and has a role in regulation cell shape. In addition S1P acts as an intracellular mediator in a receptor-independent manner regulating cell survival and Ca2+ homeostasis. Sphingosine 1-phosphate biotin (S1P-biotin) is a S1P analog with natural erythro stereochemistry labeled at the terminus of the alkyl chain with biotin.Bulk quantities of this product are available. Please email echelon@echelon-inc.com for more information. Powered by Bioz See more details on BiozFeatured in Publications1) Ieronimakis N. et al. (2013). "Increased sphingosine-1-phosphate improves muscle regeneration in acutely injured mdx mice." Skelet Muscle 3(1): 20.

Sphingosine 1-phosphate (S1P) is a bioactive lysophospholipid formed by phosphorylation of sphingosine by sphingosine kinases. S1P binds to five G-protein coupled receptors designated S1P1-5 leading to cell proliferation motility and has a role in regulation cell shape. In addition S1P acts as an intracellular mediator in a receptor-independent manner regulating cell survival and Ca2+ homeostasis. Sphingosine 1-phosphate TAMRA (S1P-TAMRA) is a S1P analog with natural erythro stereochemistry labeled at the terminus of the alkyl chain with tetramethylrhodamine.Excitation/Emission: 555/580 nmBulk quantities of this product are available. Please email echelon@echelon-inc.com for more information.Featured in Publications1) Kashem M. A. et al. (2016). "A High-Throughput Genetic Complementation Assay in Yeast Cells Identified Selective Inhibitors of Sphingosine Kinase 1 Not Found Using a Cell-Free Enzyme Assay." Assay Drug Dev Technol 14(1): 36-49.2) Nagatake T. et al. (2020). "Selecti

S1P Lyase Fluorogenic Substrate is designed for monitoring S1PL activity by releasing fluorescent 7-hydroxycoumarin in the presence of active enzyme.Sphingosine-1-phosphate lyase (S1PL) cleaves sphingosine-1-phosphate into hexadecenal and phosphoethanolamine. S1PL has been proposed as a target for autoimmune disorder treatment and in cancer therapy. Powered by Bioz See more details on BiozReferences1. Billich A. et al. (2013). "Partial deficiency of sphingosine-1-phosphate lyase confers protection in experimental autoimmune encephalomyelitis." PLoS One 8(3): e59630.2. Engel N. et al. (2012). "Metabolic profiling reveals sphingosine-1-phosphate kinase 2 and lyase as key targets of (phyto-) estrogen action in the breast cancer cell line MCF-7 and not in MCF-12A." PLoS One 7(10): e47833.3. Bedia C. et al. (2009). "Synthesis of a fluorogenic analogue of sphingosine-1-phosphate and its use to determine sphingosine-1-phosphate lyase activity." Chembiochem 10(5): 820-2.

Ceramides are lipid second messengers with an important role in apoptosis.  Biotin-Ceramide can be bound to streptavidin coated surfaces for protein binding experiments and assay development. Powered by Bioz See more details on Bioz

Sphingomyelin is a lipid constituent in the exoplasmic face of cell membranes. Sphingomyelin is hydrolyzed by Sphingomyelinases to the proapoptotic messenger Ceramide and phosphorylcholine. Biotin Sphingomyelin can be bound to streptavidin coated surfaces for protein binding experiments and assay development.Featured in Publications:Back M. J. et al. (2018). "Activation of neutral sphingomyelinase 2 by starvation induces cell-protective autophagy via an increase in Golgi-localized ceramide." Cell Death & Disease 9(6): 670.

Ceramide 1-phosphate (C1P) is biosynthesized from ceramide by the intracellular enzyme Ceramide kinase. C1P inhibits cell apoptosis via inhibition of acid sphingomyelinase activity in contrast to ceramide which promotes apoptosis. C1P is important in inflammation activating cytosolic phospholipase A2 alpha and releasing downstream inflammatory mediators. In addition C1P is involved in DNA synthesis mast cell degranulation and neutrophil phagocytosis.