DNA & Clones

The Ub-CHOP2-Reporter Deubiquitylation Assay consists of ubiquitin fused to a reporter enzyme, as well as a separate reagent substrate for the reporter enzyme. When fused to ubiquitin, the reporter is rendered catalytically inactive. Following cleavage of the Ub-reporter system by the isopeptidase, the free (and now active) reporter subsequently acts upon its substrate. Thus, in this coupled assay, the signal generated by cleavage of the reporter enzyme’s substrate is a quantitative measure of isopeptidase activity.-\n<h3><strong>Contents</strong></h3>-\n-- Ub-CHOP2-Reporter System: 1 x 375 &#0956;L (4 &#0956;M)-\n-- USP2 (Control Isopeptidase): 2 x 10 &#0956;L (2 &#0956;M)-\n-- Reporter Substrate 2: 1 x 30 &#0956;L (25 &#0956;M)-\n-- Control Fluorophore 2: 1 x 20 &#0956;L (1 mM)

The NEDD8-CHOP-Reporter DeNEDDylation Assay Kit consists of NEDD8 fused to a reporter enzyme and the reporter enzyme substrate. When fused to NEDD8, the reporter is rendered catalytically inactive. Following cleavage of the NEDD8-reporter system by isopeptidase activity, the free (and now active) reporter enzyme subsequently acts upon its substrate. Thus, in this coupled assay, the signal generated by cleavage of the reporter enzyme’s substrate is a quantitative measure of isopeptidase activity.-\n<h3><strong>Contents</strong></h3>-\n-- NEDD8-CHOP2-Reporter (Reporter System): 1 x 375 &#0956;L (4 &#0956;M)-\n-- Den1 (Control Isopeptidase): 2 x 10 &#0956;L (2 &#0956;M)-\n-- Reporter Substrate 2: 1 x 30 &#0956;L (25 &#0956;M)-\n-- Control Fluorophore 2: 1 x 20 &#0956;L (1 mM)

The ISG15-CHOP2-Reporter DeISGylation Assay consists of ISG15 fused to a reporter enzyme, as well as a separate reagent substrate for the reporter enzyme. When fused to ISG15, the reporter is rendered catalytically inactive. Following cleavage of the ISG15-reporter system by the isopeptidase, the free (and now active) reporter subsequently acts upon its substrate. Thus, in the coupled assay, the signal generated by cleavage of the reporter enzyme’s substrate is a quantitative measure of isopeptidase activity.-\n<h3><strong>Contents</strong></h3>-\n-- ISG15-CHOP2-Reporter System: 1 x 375 &#0956;L (4 &#0956;M)-\n-- PLpro (Control Isopeptidase): 2 x 10 &#0956;L (2 &#0956;M)-\n-- Reporter Substrate 2: 1 x 30 &#0956;L (25 &#0956;M)-\n-- Control Fluorophore 2: 1 x 20 &#0956;L (1 mM)

This product is available under license conditions, please take note of the Limited Use Label License in the Ressources section.

This product is available under license conditions, please take note of the Limited Use Label License in the Ressources section.

Salmon Sperm DNA was shared by sonication.DNA is dissolved in deionized distilled water, with a fragment size range of 300-700 base pairs.