DNA & Clones

DNA was prepared from cell line WM1862. WM1862 is a non-tumorigenic (RGP) primary human melanoma cell line without metastatic capability. This cell line displays pigmented, mesenchymal and spherical morphology. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. These cells expresses wild type N-RAS, c-KIT, and CDK4. WM1862 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM1862. WM1862 is a non-tumorigenic (RGP) primary human melanoma cell line without metastatic capability. This cell line displays pigmented, mesenchymal and spherical morphology. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. These cells expresses wild type N-RAS, c-KIT, and CDK4. WM1862 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM1960. WM1960 is a metastatic human melanoma cell line. This cell line contains a Q61K mutation at position 61 in the N-RAS gene which causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation. WM1960 cells are from same patient as WM2013 and WM3060. WM1960 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM1960. WM1960 is a metastatic human melanoma cell line. This cell line contains a Q61K mutation at position 61 in the N-RAS gene which causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation. WM1960 cells are from same patient as WM2013 and WM3060. WM1960 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM1960. WM1960 is a metastatic human melanoma cell line. This cell line contains a Q61K mutation at position 61 in the N-RAS gene which causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation. WM1960 cells are from same patient as WM2013 and WM3060. WM1960 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM1963. WM1963 is a human melanoma cell line that displays a melanocytic morphology and was established from a tissue sample of a 56-year-old male patient. This cell line contains a N581S mutation in BRAF gene resulting in an amino acid substitution from asparagine to serine at codon 581 (Asn581Ser) and A146T mutation in N-RAS gene resulting in a substitution of alanine with threonine at codon 146 (Ala146Thr). WM1963 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM1963. WM1963 is a human melanoma cell line that displays a melanocytic morphology and was established from a tissue sample of a 56-year-old male patient. This cell line contains a N581S mutation in BRAF gene resulting in an amino acid substitution from asparagine to serine at codon 581 (Asn581Ser) and A146T mutation in N-RAS gene resulting in a substitution of alanine with threonine at codon 146 (Ala146Thr). WM1963 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM1963. WM1963 is a human melanoma cell line that displays a melanocytic morphology and was established from a tissue sample of a 56-year-old male patient. This cell line contains a N581S mutation in BRAF gene resulting in an amino acid substitution from asparagine to serine at codon 581 (Asn581Ser) and A146T mutation in N-RAS gene resulting in a substitution of alanine with threonine at codon 146 (Ala146Thr). WM1963 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM1985. WM1985 is a human melanoma cell line that displays flat cell morphology and forms tight colonies in culture medium. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line also expresses PTEN loss of function including homozygous deletion, and is wild type for N-RAS, c-KIT, and CDK4. WM1985 cells produce xenograft tumors when injected into immunocompromised mice.