DNA & Clones

DNA was prepared from cell line WM1985. WM1985 is a human melanoma cell line that displays flat cell morphology and forms tight colonies in culture medium. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line also expresses PTEN loss of function including homozygous deletion, and is wild type for N-RAS, c-KIT, and CDK4. WM1985 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM1985. WM1985 is a human melanoma cell line that displays flat cell morphology and forms tight colonies in culture medium. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line also expresses PTEN loss of function including homozygous deletion, and is wild type for N-RAS, c-KIT, and CDK4. WM1985 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM2013. WM2013 is a metastatic human melanoma cell line that displays a melanocytic morphology. This cell line contains a Q61K mutation at position 61 in the N-RAS gene which causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation. WM2013 cells are the recurrent lesion to WM1960 and match WM3060. WM2013 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM2013. WM2013 is a metastatic human melanoma cell line that displays a melanocytic morphology. This cell line contains a Q61K mutation at position 61 in the N-RAS gene which causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation. WM2013 cells are the recurrent lesion to WM1960 and match WM3060. WM2013 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM2013. WM2013 is a metastatic human melanoma cell line that displays a melanocytic morphology. This cell line contains a Q61K mutation at position 61 in the N-RAS gene which causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation. WM2013 cells are the recurrent lesion to WM1960 and match WM3060. WM2013 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM2032. WM2032 is a metastatic human melanoma cell line that was established from a lymph node metastatic site of a patient. This cell line contains a Q61R mutation at position 61 in the N-RAS gene. The Q61R is the most common NRAS mutation found in melanoma that is thought to occur due to UV and radiation exposure. This mutation leads to production of a constitutively active N-RAS protein that directs cells to grow and divide constantly. WM2032 cells match WM3000. These cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM2032. WM2032 is a metastatic human melanoma cell line that was established from a lymph node metastatic site of a patient. This cell line contains a Q61R mutation at position 61 in the N-RAS gene. The Q61R is the most common NRAS mutation found in melanoma that is thought to occur due to UV and radiation exposure. This mutation leads to production of a constitutively active N-RAS protein that directs cells to grow and divide constantly. WM2032 cells match WM3000. These cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM2032. WM2032 is a metastatic human melanoma cell line that was established from a lymph node metastatic site of a patient. This cell line contains a Q61R mutation at position 61 in the N-RAS gene. The Q61R is the most common NRAS mutation found in melanoma that is thought to occur due to UV and radiation exposure. This mutation leads to production of a constitutively active N-RAS protein that directs cells to grow and divide constantly. WM2032 cells match WM3000. These cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM2044. WM2044 is a metastatic human melanoma cell line that displays mesenchymal morphology. These cells require insulin for growth in culture. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line also expresses PTEN loss of function including hemizygous deletion, and is wild type for N-RAS, c-KIT, and CDK4. WM2044 cells produce xenograft tumors when injected into immunocompromised mice.