DNA & Clones

DNA was prepared from cell line WM278. WM278 is a tumorigenic (VGP) primary melanoma cell line with competence for metastasis. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line also expresses PTEN loss of function including hemizygous PTEN deletion and is wild type for N-RAS, c-KIT, and CDK4. WM278 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM278. WM278 is a tumorigenic (VGP) primary melanoma cell line with competence for metastasis. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line also expresses PTEN loss of function including hemizygous PTEN deletion and is wild type for N-RAS, c-KIT, and CDK4. WM278 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3000. WM3000 is a metastatic human melanoma cell line that displays a melanocytic morphology. This cell line contains a Q61R mutation at position 61 in the N-RAS gene. The Q61R is the most common NRAS mutation found in melanoma that is thought to occur due to UV and radiation exposure. This mutation leads to production of a constitutively active N-RAS protein that directs cells to grow and divide constantly. WM3000 cells are from same patient as WM 2032. These cells are expected to produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3000. WM3000 is a metastatic human melanoma cell line that displays a melanocytic morphology. This cell line contains a Q61R mutation at position 61 in the N-RAS gene. The Q61R is the most common NRAS mutation found in melanoma that is thought to occur due to UV and radiation exposure. This mutation leads to production of a constitutively active N-RAS protein that directs cells to grow and divide constantly. WM3000 cells are from same patient as WM 2032. These cells are expected to produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3000. WM3000 is a metastatic human melanoma cell line that displays a melanocytic morphology. This cell line contains a Q61R mutation at position 61 in the N-RAS gene. The Q61R is the most common NRAS mutation found in melanoma that is thought to occur due to UV and radiation exposure. This mutation leads to production of a constitutively active N-RAS protein that directs cells to grow and divide constantly. WM3000 cells are from same patient as WM 2032. These cells are expected to produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3060. WM3060 is a metastatic human melanoma cell line established from a metastatic site (left axillary nodes) in a 55-year-old male with level III malignant melanoma. The subject displayed VGP, and RGP with a tumor thickness of 3.0 mm. This cell line was derived from the same patient as the cell lines WM2013, and WM1960. This cell line contains a Q61K mutation at position 61 in the N-RAS gene which causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation. This cell line is wild type for BRAF, PTEN, c-KIT, and CDK4. WM3060 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3060. WM3060 is a metastatic human melanoma cell line established from a metastatic site (left axillary nodes) in a 55-year-old male with level III malignant melanoma. The subject displayed VGP, and RGP with a tumor thickness of 3.0 mm. This cell line was derived from the same patient as the cell lines WM2013, and WM1960. This cell line contains a Q61K mutation at position 61 in the N-RAS gene which causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation. This cell line is wild type for BRAF, PTEN, c-KIT, and CDK4. WM3060 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3060. WM3060 is a metastatic human melanoma cell line established from a metastatic site (left axillary nodes) in a 55-year-old male with level III malignant melanoma. The subject displayed VGP, and RGP with a tumor thickness of 3.0 mm. This cell line was derived from the same patient as the cell lines WM2013, and WM1960. This cell line contains a Q61K mutation at position 61 in the N-RAS gene which causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation. This cell line is wild type for BRAF, PTEN, c-KIT, and CDK4. WM3060 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3061. WM3061 is a metastatic human melanoma cell line. These are small spherical cells that float easily after splitting and require 5% medium to adhere to culture flask. WM3061 cells produce xenograft tumors when injected into immunocompromised mice. This cell line contains a G13R mutation at position 13 in the N-RAS gene. Mutations affecting codon 13 lead to constitutive activation of RAS GTPase and ultimately neoplastic growth. The G13R is a rare NRAS mutation found in melanoma.