DNA & Clones

DNA was prepared from cell line WM3506. WM3506 is a human metastatic melanoma cell line that displays a mesenchymal morphology. This cell line contains a Q61R mutation at position 61 in the N-RAS gene. The Q61R is the most common NRAS mutation found in melanoma that is thought to occur due to UV and radiation exposure. This mutation leads to production of a constitutively active N-RAS protein that directs cells to grow and divide constantly. WM3506 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3506. WM3506 is a human metastatic melanoma cell line that displays a mesenchymal morphology. This cell line contains a Q61R mutation at position 61 in the N-RAS gene. The Q61R is the most common NRAS mutation found in melanoma that is thought to occur due to UV and radiation exposure. This mutation leads to production of a constitutively active N-RAS protein that directs cells to grow and divide constantly. WM3506 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3533. WM3533 is a human metastatic melanoma cell line that displays a fibroblastic morphology. This cell line was established from a lymph node metastases of a patient. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. WM3533 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3533. WM3533 is a human metastatic melanoma cell line that displays a fibroblastic morphology. This cell line was established from a lymph node metastases of a patient. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. WM3533 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3540. WM3540 is a human metastatic melanoma cell line that displays a pigmented, mesenchymal morphology. This cell line was established from a lymph node metastases of a patient. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line also expresses D97H mutation in CDK4 gene and is wild type for N-RAS and c-KIT. WM3540 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3540. WM3540 is a human metastatic melanoma cell line that displays a pigmented, mesenchymal morphology. This cell line was established from a lymph node metastases of a patient. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line also expresses D97H mutation in CDK4 gene and is wild type for N-RAS and c-KIT. WM3540 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3540. WM3540 is a human metastatic melanoma cell line that displays a pigmented, mesenchymal morphology. This cell line was established from a lymph node metastases of a patient. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line also expresses D97H mutation in CDK4 gene and is wild type for N-RAS and c-KIT. WM3540 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3618. WM3618F is a human metastatic uveal melanoma cell line displaying melanocytic morphology. These cells require 10% FBS to adhere to culture flask. This cell line was established from a lymph node metastases arising from uveal melanoma of a patient. Uveal melanoma is a rare type of cancer of an eye and can arise in the anterior (iris) or the posterior (ciliary body or choroid) uveal tract. Most uveal tract melanomas originate in the choroid. The ciliary body is less commonly a site of origin, and the iris is the least common. WM3618F cells match with WM3772F. These cells produce xenograft tumors when injected into immunocompromised mice.