DNA & Clones

DNA was prepared from cell line WM3755. WM3755 is a metastatic human melanoma cell line that displays a mesenchymal morphology. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line expresses wild type N-RAS, c-KIT, and CDK4 genes. WM3755 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3755. WM3755 is a metastatic human melanoma cell line that displays a mesenchymal morphology. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line expresses wild type N-RAS, c-KIT, and CDK4 genes. WM3755 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3758. WM3758 is a metastatic human melanoma cell line. This cell line contains a Q61L mutation at position 61 in the N-RAS gene. The Q61L mutation results in an amino acid substitution at position 61 in NRAS, from a glutamine (Q) to a leucine (L). This cell line expresses wild type BRAF, c-KIT, and CDK4 genes. WM3758 cells match with WM3682 and WM3702. These cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3758. WM3758 is a metastatic human melanoma cell line. This cell line contains a Q61L mutation at position 61 in the N-RAS gene. The Q61L mutation results in an amino acid substitution at position 61 in NRAS, from a glutamine (Q) to a leucine (L). This cell line expresses wild type BRAF, c-KIT, and CDK4 genes. WM3758 cells match with WM3682 and WM3702. These cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3758. WM3758 is a metastatic human melanoma cell line. This cell line contains a Q61L mutation at position 61 in the N-RAS gene. The Q61L mutation results in an amino acid substitution at position 61 in NRAS, from a glutamine (Q) to a leucine (L). This cell line expresses wild type BRAF, c-KIT, and CDK4 genes. WM3758 cells match with WM3682 and WM3702. These cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3772. WM3772F is a primary human metastatic uveal melanoma cell line displaying clumpy pigmented morphology. These cells require 5% FBS to adhere to culture flask. This cell line was established from a metastases arising from uveal melanoma of a patient. Uveal melanoma is a rare type of cancer of an eye and can arise in the anterior (iris) or the posterior (ciliary body or choroid) uveal tract. Most uveal tract melanomas originate in the choroid. The ciliary body is less commonly a site of origin, and the iris is the least common. This cell line expresses wild type BRAF, N-RAS, c-KIT, and CDK4 genes. WM3772F cells match with WM3618F. These cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3772. WM3772F is a primary human metastatic uveal melanoma cell line displaying clumpy pigmented morphology. These cells require 5% FBS to adhere to culture flask. This cell line was established from a metastases arising from uveal melanoma of a patient. Uveal melanoma is a rare type of cancer of an eye and can arise in the anterior (iris) or the posterior (ciliary body or choroid) uveal tract. Most uveal tract melanomas originate in the choroid. The ciliary body is less commonly a site of origin, and the iris is the least common. This cell line expresses wild type BRAF, N-RAS, c-KIT, and CDK4 genes. WM3772F cells match with WM3618F. These cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3772. WM3772F is a primary human metastatic uveal melanoma cell line displaying clumpy pigmented morphology. These cells require 5% FBS to adhere to culture flask. This cell line was established from a metastases arising from uveal melanoma of a patient. Uveal melanoma is a rare type of cancer of an eye and can arise in the anterior (iris) or the posterior (ciliary body or choroid) uveal tract. Most uveal tract melanomas originate in the choroid. The ciliary body is less commonly a site of origin, and the iris is the least common. This cell line expresses wild type BRAF, N-RAS, c-KIT, and CDK4 genes. WM3772F cells match with WM3618F. These cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3792. WM3792 is a metastatic human melanoma cell line that displays a dendritic morphology. This cell line contains V600K (Val600Lys) mutation at codon 600 in the BRAF gene. This mutation occurs within the activation segment of the kinase domain and causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line also expresses a novel mutation––the deletion of codon 601, and contains a PTEN loss of function including homozygous deletion. WM3792 cells produce xenograft tumors when injected into immunocompromised mice.