Enzymes

The USP8 Inhibitor Screening Assay Kit is a fluorogenic assay designed to measure the activity of the deubiquitinating (DUB) enzyme USP8 (ubiquitin specific peptidase 8) for screening and profiling applications. The kit comes in a convenient 96-well format and contains enough purified recombinant human USP8 protein, Ubiquitinated-AMC substrate, and assay buffer for 100 reactions. To determine the effect of an inhibitor on USP8 activity the enzyme should be preincubated with or without the test inhibitor prior to adding the Ub-AMC substrate to the reaction. The assay was functionally validated using Ub-Aldehyde, a potent inhibitor of DUB subfamilies Ubiquitin C-terminal Hydrolases (UCHs), Ubiquitin-Specific Proteases (USPs), Ovarian Tumor Proteases (OTU), and Machado-Josephin Domain (MJD) proteases. Ubiquitin-AMC (Ub-AMC) is a fluorogenic substrate for ubiquitin hydrolases, based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC). In the conjugated form th

Human MTM1 full-length ORF (Q13496, 1 a.a. - 603 a.a.) recombinant protein with HIS tag at N-terminal.

Human PROS1 full-length ORF (P07225, 42 a.a. - 676 a.a.) recombinant protein with HIS-SUMO tag at N-terminal.

Human FOXD1 partial ORF (NP_004463.1, 1 a.a. - 90 a.a.) recombinant protein with GST tag at N-terminal.

Human FOXD1 partial ORF (NP_004463.1, 116 a.a. - 232 a.a.) recombinant protein with GST tag at N-terminal.

Human FOXC2 partial ORF (NP_005242.1, 156 a.a. - 256 a.a.) recombinant protein with GST tag at N-terminal.

Recombinant human PI3 Kinase complex of p110α, with an E542K mutation, and p85α variant 1. Subunit p110α encompasses amino acids 2-1068(end), and an N-terminal FLAG tag. Subunit p85α encompasses amino acids 1-724(end). The two constructs were expressed together, and the complex was affinity purified.

Recombinant human PI3 Kinase complex of p110α, with an H1047L mutation, and p85α variant 1. Subunit p110α encompasses amino acids 2-1068(end), and an N-terminal FLAG tag. Subunit p85α encompasses amino acids 1-724(end). The two constructs were expressed together, and the complex was affinity purified.

Recombinant human ALK (C1156Y) (anaplastic lymphoma kinase), encompassing amino acids 1060-end with a C1156Y mutation. This construct contains an N-terminal GST-tag. The recombinant protein was affinity purified and is active.