Chemicals & Small Molecules

SiR-tetrazine is a clickable derivative of SiR. This probe reacts with dienophiles such as trans-cyclooctene (TCO) or bicyclononyne (BCN) derivatives, enabling the preparation of custom SiR probes and conjugates. SiR-tetrazine is particularly useful in combination with unnatural amino acid (UAA) incorporation to prepare site selective protein conjugates. SiR-tetrazine is cell permeable and fluorogenic, it can be used to stain live cells as well as fixed ones. SiR is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with a standard Cy5 filterset.

SiR-azide is the 3-azidopropyl derivative of the fluorophore silicon rhodamine (SiR). Sir-azide reacts with terminal alkyne derivatives in the presence of copper (I) to form 1,2,3-triazoles linkage. It can also react with strained alkynes such as DIFO or DIBO without the need of copper. Small molecules, peptides, oligonucleotides or proteins bearing the appropriate alkyne can be conjugated to SiR-azide. SiR-azide is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with a standard Cy5 filterset.

SiR-alkyne is the N-propargylcarboxamide derivative of the fluorophore silicon rhodamine (SiR). Sir-alkyne reacts with azide derivatives in the presence of copper (I) to form 1,2,3-triazoles linkage. Small molecules, peptides, oligonucleotides or proteins bearing the appropriate azide can be conjugated to SiR-alkyne. SiR excitation and emission are compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with a standard Cy5 filterset.

SiR-maleimide is the 2-maleimidoethyl derivative of the fluorophore silicon rhodamine (SiR). SiR-maleimide reacts with thiols to form covalent conjugates. Small molecules, peptides, oligonucleotides or proteins bearing a free thiol can be conjugated to SiR-maleimide. SiR excitation and emission are compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with a standard Cy5 filterset.

SiR-lysosome is based on the cathepsin D binding natural product pepstatin A. SiR-lysosome is fluorogenic, cell permeable and highly specific for lysosomes. Sir-lysosome stains lysosomes in ilve cells without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR-lysosome is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with standard Cy5 filtersets. SiR-lysosome can be used for widefield, confocal, SIM or STED imaging in living cells and tissue.

SiR700-actin is based on the F-actin binding natural product jasplakinolide. SiR700-actin is fluorogenic, cell permeable and highly specific for F-actin. SiR700-actin stains endogenous F-actin without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR700-actin is compatible with GFP and/or m-cherry fluorescent proteins. SiR700-actin can be used for widefield, confocal, SIM or STED imaging in living cells and tissue.

SiR700-tubulin is based on the microtubule binding drug docetaxel. SiR700-tubulin is fluorogenic, cell permeable and highly specific for microtubules. SiR700-tubulin stains endogenous microtubules without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR-tubulin is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with standard Cy5 filtersets. SiR700-tubulin can be used for widefield, confocal, SIM or STED imaging in living cells and tissue.

SiR700-DNA is based on the DNA minor groove binder bisbenzimide, it allows the labelling of DNA in live cells with high specificity and low background. SiR700-DNA is fluorogenic, cell permeable and highly specific for DNA. SiR700-DNA stains the nuclei of live cells without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR700-DNA is compatible with GFP and/or m-cherry fluorescent proteins and is a non cytotoxic alternative to DRAQ5. It can be imaged with standard Cy5 filtersets. SiR700-DNA can be used for widefield, confocal, SIM or STED imaging in living cells and tissue

SiR700-lysosome is based on the cathepsin D binding natural product pepstatin A. SiR700-lysosome is fluorogenic, cell permeable and highly specific for lysosomes. SiR700-lysosome stains lysosomes in ilve cells without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR700-lysosome is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with standard Cy5 filtersets. SiR700-lysosome can be used for widefield, confocal, SIM or STED imaging in living cells and tissue.