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The Human Bead-based Immuno-Quantitative (BIQ) CD27 Ligand/TNFSF7 ELISA Kit merges the specificity of sandwich-based ELISA, the sensitivity of real-time PCR, and the easy manipulation of microspheres to detect even the lowest levels of CD27 Ligand/TNFSF7 in serum, plasma, and cell culture supernatant samples.