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The Human Bead-based Immuno-Quantitative (BIQ) TNF-beta/LTA/TNFSF1B ELISA Kit merges the specificity of sandwich-based ELISA, the sensitivity of real-time PCR, and the easy manipulation of microspheres to detect even the lowest levels of TNF-beta/LTA/TNFSF1B in serum, plasma, and cell culture supernatant samples.