Bioassays
With a combination of validated Biochemical and more physiologically relevant assays we can help you to decipher and evaluate:
- Mechanism Of Action (MoA) of your hits
- Inhibitors (e.g. immune checkpoints, enzymatic assays, signalling pathway...)
- Activators (e.g. ADCC, enzymatic assays, signalling pathways...)
- Blocking effects (SARS CoV 2…), synergistic effects
- in Vitro toxicology screening
- Target selectivity & efficiency
- Broader effects by combining our other technical platforms
- Impact on biomarker expression (Biomarker platform)
- Phenotypic effects (Cell Culture platform)
- From model culturing & treatment to read-out analysis
Your benefits
- Wide range of possibilities through our combinable service platforms
- Reduced costs with reagents from our broad catalogue
- Our in-house experience, reproducibility and data quality
- Regular contact throughout with your designated project manager
Discover some Bioassay case studies
Our solution
We performed comparative analysis for them, which involved developing two HT-29 colorectal cancer models: as monolayers in a conventional incubator and as 3D spheroids under physioxia, in the presence/absence of gamma-interferon.
On each model, a viability assay was performed after 3 days incubation time with inhibitors, followed by large-scale proteome profiling analysis on 2000 targets from each cell lysates.
Our solution:
We established and implemented a physiologically relevant 3D Colorectal cancer cell-based assay. To answer questions related to variations at transcription and protein level, our methodology required long-term imaging at controlled temperature and gas levels (CO2 and O2), on both 3D cellular models and patient-derived samples. Impact of an anti-cancerous drug was investigated on colon cell lines (HT-29, HCT116 and LS174T) in conventional 2D monolayer and 3D spheroid cultures, in hypoxia or normoxia. Methods used were metastatic invasion assay, gene expression analysis by RT-PCR on hypoxic response and oncopathway genes, and large-scale protein profiling to detect proteomic levels of metalloproteinases and surface proteins known to be involved in colorectal cancer invasion.
