Cell-based Assays

Sucrose is the primary form in which photosynthetic products from sources (such as leaves) are transported to “sink” organs. Sucrose synthase (EC 2.4.1.13) is a reversible enzyme that can catalyze both the synthesis and breakdown of sucrose, making it a key enzyme in sucrose metabolism. Studying the activity of its invertase direction (SS-I) is significant for understanding plant sucrose degradation and starch synthesis. CheKine™ Mirco Sucrose Synthase-I (SS-I) Activity Assay Kit offers a simple, convenient, and rapid approach for detecting sucrose synthase (SS-I) activity, suitable for plant tissue samples. The principle of the kit involves SS-I catalyzing the reaction between sucrose and UDP to produce free fructose and UDPG; the content of reducing sugars is then measured using the 3,5-dinitrosalicylic acid method to reflect the level of enzyme activity

Sucrase (EC 3.2.1.26) is one of the key enzymes for carbohydrate digestion and absorption, capable of hydrolyzing sucrose into monosaccharides that can be absorbed by the body. CheKine™ Mirco Sucrase Activity Assay Kit offers a simple, convenient, and rapid approach for assessing sucrase activity, which is suitable for plant tissue samples. The principle is that 3,5-dinitrosalicylic acid, when heated with reducing sugars, is reduced to a brown-red amino compound, and within a certain range, the quantity of reducing sugars is proportional to the color intensity of the reaction solution. This method is easy to perform, quick, and has minimal interference from impurities.

All-in-one kit for making phase-separated droplets with BSA (Bovine Serum Albumin), Droplets are easy to observe using only a micropipette and microscope, Includes a user's manual with calculation examples and details on how to use it

Set of reagents necessary to confirm the conditions for making phase-separated droplets, Crowding agent, pH, and salt can be optimized with this kit, Includes user's manual with sample calculations and methods for adjusting buffer pH

Detect SA-β-Gal levels, Compatible with co-staining with immunostaining, Enable multiple staining with common green and red dyes

Easy identification of cell death pathways in combination with other Dojindo’s kits, Multiple indicators can be measured in combination on the same plate, and Accurate plate assay without the need for washing.

The RayBio® Human TBMNK Flow Cytometry Kit is designed to determine the percentages of mature human lymphocyte and monocyte subsets in peripheral whole blood (WB).

The RayBio® Human TBMNK Flow Cytometry Kit is designed to determine the percentages of mature human lymphocyte and monocyte subsets in peripheral whole blood (WB).

Measure Gi signaling kinetics or activation with this live cell cAMP assay. This is a update to #X0200G, packaged in Big Sky BacMam providing a 3-4x increase in expression in cell types including HEK293 and CHO cells. BacMam also robustly transduces many iPSC and primary cultures, including neuronal and cardiac cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (D2R BacMam and 2mM quinpirole in H2O). Other Montana Molecular tools including BacMam-packaged GPCRs can easily be co-expressed with this assay. 15mL and 30mL sizes are also available.