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The 2 x Taq PCR Mix with MgCl2 is a premixed solution containing everything needed for a successful PCR reaction except specific primers and DNA templates. The mix includes high-quality recombinant Taq DNA polymerase, nucleotides, and magnesium in a PCR reaction buffer. For the reaction set-up add the PCR Mix (10 or 25 uL) to the primers, template, and water for the total reaction volume of 20 or 50 uL.